Mouse p23 Monoclonal Antibody | anti-PTGES3 antibody

p23 Antibody

Cat. Num. AAA803474

• Gene Names: PTGES3; P23; TEBP; cPGES
• Reactivity: Human, Mouses, Rabbit, Chicken, Guinea Pig, S. cerevisiae (lower)
• Applications: Western Blot, Immunoprecipitation, ELISA
• Synonyms: p23; Monoclonal Antibody; p23 Antibody; co chaperone p23; PTGES3; TEBP; telomerase binding protein p23; unactive progesterone receptor 23kDa; Hsp90 co-chaperone; Progesterone receptor complex p23; Cytosolic prostaglandin E2 synthase; anti-PTGES3 antibody

• Host: Mouse
• Reactivity: Human, Mouses, Rabbit, Chicken, Guinea Pig, S. cerevisiae (lower)
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: JJ6
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-PTGES3 antibody: Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: 1:2000 (WB)
• Certificate of Analysis: 0.5ug/mL was sufficient for detection of p23 in 20ug of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining.)

Western Blot (WB)

(Western Blot analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining - some evidence of cell-cell borders.)

Related Product Information for anti-PTGES3 antibody

Background Info: Detects ~23kDa.

Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the Hsp90-progesterone receptor complex along with hsp70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate-mediated hsp90 binding of client proteins (8). Since many hsp90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). Hsp90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.

Product Categories/Family for anti-PTGES3 antibody

Chaperones; Heat Shock; Trafficking

References

1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. & Toft, D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237- 50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones 8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia.
1. Shimamura, T. et al. (2012). Ganetespib (STA-9090), a Nongeldanamycin HSP90 Inhibitor, Has Potent Antitumour Activity in In Vitro and In Vivo Models of Non-Small Cell Lung Cancer. Clin Cancer Res. 18, 4973-4985. doi: 10.1158/1078-0432.CCR-11-2967

NCBI and Uniprot Product Information

• NCBI GI #: 23308579
• NCBI GeneID: 10728
• NCBI Accession #: NP_006592.3
• NCBI GenBank Nucleotide #: NM_006601.6
• UniProt Accession #: Q15185; Q8WU70; A8K7D0; B4DHP2; B4DP11; B4DP21
• Molecular Weight: 16,476 Da
• NCBI Official Full Name: prostaglandin E synthase 3 isoform a
• NCBI Official Synonym Full Names: prostaglandin E synthase 3 (cytosolic)
• NCBI Official Symbol: PTGES3
• NCBI Official Synonym Symbols: P23; TEBP; cPGES
• NCBI Protein Information: prostaglandin E synthase 3; Hsp90 co-chaperone; telomerase-binding protein p23; progesterone receptor complex p23; cytosolic prostaglandin E synthase; cytosolic prostaglandin E2 synthase; unactive progesterone receptor, 23 kD
• UniProt Protein Name: Prostaglandin E synthase 3
• Protein Family: Prostaglandin E synthase
• UniProt Gene Name: PTGES3
• UniProt Synonym Gene Names: P23; TEBP; cPGES
• UniProt Entry Name: TEBP_HUMAN

Uniprot Description

TEBP: Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor- mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Belongs to the p23/wos2 family.

Protein type: EC 5.3.99.3; Nuclear receptor co-regulator; Chaperone; Transferase; Isomerase

Chromosomal Location of Human Ortholog: 12q13.3|12

Cellular Component: nucleoplasm; chromosome, telomeric region; telomerase holoenzyme complex; cytoplasm; nucleus; cytosol

Molecular Function: telomerase activity; protein binding; unfolded protein binding; prostaglandin-E synthase activity

Biological Process: RNA-dependent DNA replication; cyclooxygenase pathway; arachidonic acid metabolic process; signal transduction; prostaglandin biosynthetic process; telomere maintenance