Mouse anti-Human MYD88 Monoclonal Antibody | anti-MYD88 antibody

Anti-MYD88 Antibody (clone 1B4) IHC-plus

Cat. Num. AAA249490

• Gene Names: MYD88; MYD88D
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G purified
• Synonyms: MYD88; Monoclonal Antibody; Anti-MYD88 Antibody (clone 1B4) IHC-plus; Mouse Monoclonal [clone 1B4] (IgG2a) to Human MYD88; Human MYD88; MYD88D; anti-MYD88 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1B4
• Specificity: Human MYD88
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 0.02% sodium azide, 1% BSA, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-MYD88 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); IF (1:100); IHC-P (10 ug/ml); WB (1:2000); ; Optimal dilution to be determined by the researcher.
• Immunogen: MYD88 antibody was raised against full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
• Immunogen Description: Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MYD88 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MYD88 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Anti-MYD88 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody, and then analyzed by flow cytometry.)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-MYD88 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Related Product Information for anti-MYD88 antibody

MYD88 Antibody, MYD88D Antibody Description: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation.

NCBI and Uniprot Product Information

• NCBI GI #: 197276654
• NCBI GeneID: 4615
• NCBI Accession #: NP_002459.2
• NCBI GenBank Nucleotide #: NM_002468.4
• UniProt Accession #: Q99836; P78397; Q53XS7; B4DKH8; B4DKU4; B4DQ60; B4DQ72; J3KPU4; J3KQ87; J3KQJ6
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88 isoform 2
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: MYD88
• UniProt Entry Name: MYD88_HUMAN

NCBI Description

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: cytosol; endosome membrane; plasma membrane

Molecular Function: death receptor binding; identical protein binding; protein binding

Biological Process: cell surface receptor linked signal transduction; defense response to Gram-positive bacterium; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; nerve growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-17 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; regulation of inflammatory response; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency