Typical Testing Data/Standard Curve (for reference only)

Monkey VEGFA ELISA Kit | VEGFA elisa kit

Monkey VEGFA ELISA Kit

Reactivity

Monkey

Synonyms

VEGFA; Monkey VEGFA ELISA Kit; VEGFA elisa kit

Ordering
Cat. Num. AAA9141983 For Research Use Only!

Reactivity

Monkey

Specificity

This assay has high sensitivity and excellent specificity for detection of VEGFA. No significant cross-reactivity or interference between VEGFA and analogues was observed.

Samples

Cell culture supernates, serum and plasma

Assay Type

Quantitative Sandwich

Sensitivity

< 27 pg/mL

Intra-assay Precision

Intra-plate Precision 3 samples with low, middle and high level VEGFA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Inter-assay Precision

Inter-plate Precision 3 samples with low, middle and high level VEGFA were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for VEGFA elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VEGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFA present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for VEGFA is added to the wells and binds to the combination of capture antibody VEGFA in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of VEGFA present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven VEGFA standard dilutions and VEGFA sample concentration determined.

Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Monkey VEGFA concentrations in cell culture supernates, serum and plasma.

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Product Notes

The Monkey VEGFA (Catalog #AAA9141983) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9141983 ELISA Kit recognizes Monkey VEGFA. It is sometimes possible for the material contained within the vial of "VEGFA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Monkey VEGFA ELISA Kit | VEGFA elisa kit