Mouse IFN-gamma ELISA Kit | IFNG elisa kit

Mouse IFN-gamma ELISA Kit

Cat. Num. AAA9135739

• Gene Names: IFNG; IFG; IFI
• Reactivity: Mouse
• Synonyms: IFN-gamma; Mouse IFN-gamma ELISA Kit; IFNG elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes both recombinant and natural mouse IFN-gamma. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
• Sequence Length: 166

• Assay Type: Quantitative Sandwich
• Sensitivity: < 3.9pg/mL
• Intra-assay Precision: Intra-plate Precision 3 samples with low, middle and high level IFN-gamma were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-plate Precision 3 samples with low, middle and high level IFN-gamma were tested on 3 different plates, 20 replicates in each plate. Inter-Assay: CV<15%
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNG elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for mouse IFN-gamma has been pre-coated onto a microplate.Standards and samples are pipetted into the wells and any IFN-gamma present is bound by the immobilized antibody. After washing away any unbound substances, and then a detection antibody specific for IFN-gamma is added to the wells and binds to the combination of capture antibody IFN-gamma in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gamma bound in the initial step. The color development is stopped and the absorbance is measured.

Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFN-gamma in mouse serum, plasma, cell culture supernatants, cell lysates, tissue homogenates and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 56786138
• NCBI GeneID: 3458
• NCBI Accession #: NP_000610.2
• NCBI GenBank Nucleotide #: NM_000619.3
• UniProt Accession #: P01579; Q53ZV4; B5BU88
• Molecular Weight: 19,348 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: IFNG
• NCBI Official Synonym Symbols: IFG; IFI
• NCBI Protein Information: interferon gamma
• UniProt Protein Name: Interferon gamma
• Protein Family: Interferon
• UniProt Gene Name: IFNG
• UniProt Synonym Gene Names: IFN-gamma

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Uniprot Description

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.