Typical Testing Data/Standard Curve (for reference only)
Rat N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit | NT-ProBNP elisa kit
Rat N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit (NT-ProBNP)
Gene Names
Nppb; BNP; Bnf
Reactivity
Rat
Synonyms
N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP); Rat N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit (NT-ProBNP); NT-ProBNP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Lpa. No significant cross-reactivity or interference between Lpa and analogues was observed.
Sequence Length
121
Samples
Human Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Sensitivity
1.31ng/mL
Intra-assay Precision
3 samples with low, middle and high level Lpa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
3 samples with low, middle and high level Lpa were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NT-ProBNP elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Lpa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lpa present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Lpa is added to the wells and binds to the combination of capture antibody-Lpa in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of Lpa present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven Lpa standard dilutions and Lpa sample concentration determined!!Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Lpa in human serum, plasma, tissue homogenates and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,656 Da
NCBI Official Full Name
natriuretic peptides B
NCBI Official Synonym Full Names
natriuretic peptide B
NCBI Official Symbol
Nppb
NCBI Official Synonym Symbols
BNP; Bnf
NCBI Protein Information
natriuretic peptides B
UniProt Protein Name
Natriuretic peptides B
UniProt Gene Name
Nppb
NCBI Description
hormone produced primarily by the atrium and ventricle of the heart [RGD, Feb 2006]
Uniprot Description
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 ().
Research Articles on NT-ProBNP
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Product Notes
The Rat NT-ProBNP nppb (Catalog #AAA9139382) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9139382 ELISA Kit recognizes Rat NT-ProBNP. It is sometimes possible for the material contained within the vial of "N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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