Rabbit anti-Human, Mouse ARHGDIA Polyclonal Antibody | anti-ARHGDIA antibody

ARHGDIA (N-Term) Rabbit pAb

Cat. Num. AAA8558727

• Gene Names: ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: ARHGDIA; Polyclonal Antibody; ARHGDIA (N-Term) Rabbit pAb; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e; anti-ARHGDIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.09% (W/V) Sodium Azide, pH 7.3.
• Sequence Length: 204

• Applicable Applications for anti-ARHGDIA antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1:1000; IHC-P: 1:25
• Immunogen: This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human ARHGDIA.
• Cellular Localization: Cytoplasm
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Related Product Information for anti-ARHGDIA antibody

Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.

NCBI and Uniprot Product Information

• NCBI GI #: 297374782
• NCBI GeneID: 396
• NCBI Accession #: NP_001172006.1
• NCBI GenBank Nucleotide #: NP_001172006.1
• UniProt Accession #: P52565
• Molecular Weight: 23; 23; 23kDa
• NCBI Official Full Name: rho GDP-dissociation inhibitor 1 isoform a
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor alpha
• NCBI Official Symbol: ARHGDIA
• NCBI Official Synonym Symbols: GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e
• NCBI Protein Information: rho GDP-dissociation inhibitor 1
• UniProt Protein Name: Rho GDP-dissociation inhibitor 1
• UniProt Gene Name: ARHGDIA
• UniProt Synonym Gene Names: GDIA1; Rho GDI 1
• UniProt Entry Name: GDIR1_HUMAN

NCBI Description

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.

Protein type: Cell adhesion; G protein regulator, misc.; Apoptosis; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoskeleton; immunological synapse; cytosol

Molecular Function: protein binding; Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of protein localization; nerve growth factor receptor signaling pathway; small GTPase mediated signal transduction; negative regulation of axonogenesis; positive regulation of axonogenesis; negative regulation of cell adhesion; cell motility; regulation of axonogenesis; negative regulation of cell migration; Rho protein signal transduction; negative regulation of apoptosis; positive regulation of GTPase activity

Disease: Nephrotic Syndrome, Type 8