Western Blot (WB)
(Western blot detection of RhoGDI in C6, Jurkat, K562, 3T3 and Hela cell lysates using RhoGDI mouse mAb (1:2000 diluted).Predicted band size:26KDa.Observed band size:26KDa.)
Mouse RhoGDI Monoclonal Antibody | anti-RhoGDI antibody
Anti-RhoGDI Mouse mAb
Western Blot (WB)
(Western blot detection of RhoGDI in C6, Jurkat, K562, 3T3 and Hela cell lysates using RhoGDI mouse mAb (1:2000 diluted).Predicted band size:26KDa.Observed band size:26KDa.)
Western Blot (WB)
(Western blot detection of RhoGDI in C6, Jurkat, K562, 3T3 and Hela cell lysates using RhoGDI mouse mAb (1:2000 diluted).Predicted band size:26KDa.Observed band size:26KDa.)
Western Blot (WB)
(Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.)
Western Blot (WB)
(Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).)
UniPort Summary Function: Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.
Protein type: Apoptosis; Cell adhesion; Cell development/differentiation; G protein regulator, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoskeleton; cytosol
Molecular Function: protein binding
Biological Process: cell motility; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell adhesion; positive regulation of axonogenesis; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction
Disease: Nephrotic Syndrome, Type 8
Research Articles on RhoGDI
Similar Products
Product Notes
The RhoGDI arhgdia (Catalog #AAA476324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RhoGDI Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RhoGDI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RhoGDI arhgdia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoGDI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
