Human Mannose Binding Lectin (MBL) ELISA Kit | MBL elisa kit

Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit

Cat. Num. AAA2707867

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Applications: ELISA
• Synonyms: Mannose Binding Lectin (MBL); Human Anti-Mannose Binding Lectin Antibody (Anti-MBL) ELISA Kit; Mannose Binding Lectin; MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2; Soluble(Opsonic Defect); MBL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Mannose Binding Lectin Antibody (Anti-MBL).; No significant cross-reactivity or interference between Anti-Mannose Binding Lectin Antibody (Anti-MBL) and analogues was observed.
• Sequence Length: 248

• Applicable Applications for MBL elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma and other biological fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.45ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mannose Binding Lectin Antibody (Anti-MBL) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Mannose Binding Lectin Antibody (Anti-MBL) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Mannose Binding Lectin Antibody (Anti-MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4557739
• NCBI GeneID: 4153
• NCBI Accession #: NP_000233.1
• NCBI GenBank Nucleotide #: NP_000233.1
• UniProt Accession #: P11226
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose binding lectin 2
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: MreB-like protein
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C
• UniProt Entry Name: MBL2_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency