Western Blot (WB)
(Western blot analysis of extracts of Jurkat celllines, using CFLAR antibody.)
Rabbit CFLAR Antibody | anti-CFLAR antibody
CFLAR Antibody
Gene Names
CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
CFLAR; CFLAR Antibody; CASH; CASP8AP1; CLARP; Casper; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; anti-CFLAR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
480
Applicable Applications for anti-CFLAR antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human CFLAR
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of Jurkat celllines, using CFLAR antibody.)
Western Blot (WB)
(Western blot analysis of extracts of Jurkat celllines, using CFLAR antibody.)
Related Product Information for anti-CFLAR antibody
Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis that has various names, such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice isoforms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) like those found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE), and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both FLIP isoforms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some research studies have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease, resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkin's lymphoma and ovarian and colon carcinomas (9).
Product Categories/Family for anti-CFLAR antibody
References
1. Irmler, M. et al. (1997) Nature 388, 190-195. 2. Shu, H. B. et al. (1997) Immunity 6, 751-763. 3. Inohara, N. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10717-10722. 4. Srinivasula, S. M. et al. (1997) J. Biol. Chem. 272, 18542-18545. 5. Hu, S. et al. (1997) J. Biol. Chem. 272, 17255-17257. 6. Han, D. K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 11333-11338. 7. Dita, R. M. et al. (1998) Cell Death Differ. 5, 271-288. 8. Goltsev, Y. V. et al. (1997) J. Biol. Chem. 272, 19641-19644. 9. Kataoka, T. (2005) Crit. Rev. Immunol. 25, 31-58.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52kDa
NCBI Official Full Name
CASP8 and FADD-like apoptosis regulator isoform 1
NCBI Official Synonym Full Names
CASP8 and FADD like apoptosis regulator
NCBI Official Symbol
CFLAR
NCBI Official Synonym Symbols
CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
NCBI Protein Information
CASP8 and FADD-like apoptosis regulator
UniProt Protein Name
CASP8 and FADD-like apoptosis regulator
Protein Family
UniProt Gene Name
CFLAR
UniProt Synonym Gene Names
CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
NCBI Description
The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]
Uniprot Description
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Research Articles on CFLAR
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Product Notes
The CFLAR cflar (Catalog #AAA8505196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CFLAR Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CFLAR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CFLAR cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFLAR, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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