Mouse Interleukin 27 Receptor Alpha (IL27Ra) ELISA Kit | IL27Ra elisa kit

Mouse Interleukin 27 Receptor Alpha (IL27Ra)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA150875

• Gene Names: Il27ra; CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Interleukin 27 Receptor Alpha (IL27Ra); N/A; Mouse Interleukin 27 Receptor Alpha (IL27Ra)(Sandwich ELISA) ELISA Kit; ELISA Kit for Interleukin 27 Receptor Alpha (IL27Ra); IL27Ra/Interleukin 27 Receptor Alpha; CRL1; IL27R; TCCR; WSX1; Zcytor1; T-Cell Cytokine Receptor Type 1; Cytokine receptor-like 1; Type I T-cell cytokine receptor; IL27Ra elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL27Ra. No significant cross-reactivity or interference between IL27Ra and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL

• Applicable Applications for IL27Ra elisa kit: ELISA
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL27Ra were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL27Ra were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for IL27Ra elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IL27Ra. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IL27Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL27Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL27Ra in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL27Ra in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for IL27Ra elisa kit

Metabolic pathway; Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 170932522
• NCBI GeneID: 50931
• NCBI Accession #: NP_057880.2
• NCBI GenBank Nucleotide #: NM_016671.3
• UniProt Accession #: O70394; E9QNY4
• Molecular Weight: 69,093 Da
• NCBI Official Full Name: interleukin-27 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 27 receptor, alpha
• NCBI Official Symbol: Il27ra
• NCBI Official Synonym Symbols: CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
• NCBI Protein Information: interleukin-27 receptor subunit alpha; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; T cell cytokine receptor; IL-27 receptor subunit alpha; type I T-cell cytokine receptor; cytokine receptor family, class 1 (WSXWS), member 1; T cell cytokine receptor; cyt
• UniProt Protein Name: Interleukin-27 receptor subunit alpha
• UniProt Gene Name: Il27ra
• UniProt Synonym Gene Names: Tccr; Wsx1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
• UniProt Entry Name: I27RA_MOUSE