Human RECK ELISA Kit | RECK elisa kit

Human RECK(Reversion-inducing-cysteine-rich protein with kazal motifs)ELISA Kit

Cat. Num. AAA2505382

• Gene Names: RECK; ST15
• Reactivity: Human
• Synonyms: RECK; Human RECK(Reversion-inducing-cysteine-rich protein with kazal motifs)ELISA Kit; RECK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human RECK. No significant cross-reactivity or interference between Human RECK and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human RECK and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 971

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RECK elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RECK in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RECK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human RECK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RECK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RECK. You can calculate the concentration of Human RECK in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11863156
• NCBI GeneID: 8434
• NCBI Accession #: NP_066934.1
• NCBI GenBank Nucleotide #: NM_021111.2
• UniProt Accession #: O95980; Q5W0K6; Q8WX37; B2RNS1
• Molecular Weight: 106,457 Da
• NCBI Official Full Name: reversion-inducing cysteine-rich protein with Kazal motifs
• NCBI Official Synonym Full Names: reversion-inducing-cysteine-rich protein with kazal motifs
• NCBI Official Symbol: RECK
• NCBI Official Synonym Symbols: ST15
• NCBI Protein Information: reversion-inducing cysteine-rich protein with Kazal motifs; membrane-anchored glycoprotein (metastasis and invasion); suppression of tumorigenicity 15 (reversion-inducing-cysteine-rich protein with kazal motifs); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs); suppressor of tumorigenicity 15 protein
• UniProt Protein Name: Reversion-inducing cysteine-rich protein with Kazal motifs
• Protein Family: Reversion-inducing cysteine-rich protein
• UniProt Gene Name: RECK
• UniProt Synonym Gene Names: ST15; hRECK
• UniProt Entry Name: RECK_HUMAN

NCBI Description

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

RECK: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. Interacts with MMP-9. Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.

Protein type: Membrane protein, GPI anchor; Tumor suppressor

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: membrane; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: extracellular matrix organization and biogenesis; blood vessel maturation; embryo implantation