Rabbit ATG5 Polyclonal Antibody | anti-ATG5 antibody

RABBIT ANTI ATG5

Cat. Num. AAA224013

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Bovine (Expected from Sequence), Fish (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ATG5; Polyclonal Antibody; RABBIT ANTI ATG5; anti-ATG5 antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Fish (Expected from Sequence), Mouse (Expected from Sequence), Pig (Expected from Sequence), Rat (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises Human Autophagy Related 5 Homologue (ATG5). ; ; ATG5 is an essential protein involved in the autophagy process and conjugates to ATG12. ; ; Research has also indicated that ATG5 may play an important role in apoptosis where it is expressioned late in the apoptopic process downstream of caspase activity.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: Total protein concentration 0.5mg/ml (varies by lot)
• Sequence Length: 275

• Applicable Applications for anti-ATG5 antibody: Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. ; Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.05% Sodium Azide (NaN3); 0.2% Gelatin
• Immunogen: Synthetic peptide sequence corresponding to a region between amino acids 1-50 of human ATG5 - conjugated to KLH.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to ATG5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human ATG5 (MBS224013))

Related Product Information for anti-ATG5 antibody

MBS224013 recognises Human Autophagy Related 5 Homologue (ATG5). ATG5 is an essential protein involved in the autophagy process and conjugates to ATG12. Research has also indicated that ATG5 may play an important role in apoptosis where it is expressioned late in the apoptopic process downstream of caspase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; ATG5 autophagy related 5 homolog; apoptosis-specific protein
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation