Testing Data
Mammalian Neutral Red Cell Cytotoxicity Assay Kit
Neutral Red Cell Cytotoxicity Assay Kit
Reactivity
Mammalian
Synonyms
Neutral Red Cell Cytotoxicity; Neutral Red Cell Cytotoxicity Assay Kit; Neutral Red Cell Cytotoxicity assay kit
Reactivity
Mammalian
Samples
Cell culture: Adherent cellsand non-adherent cells
Detection Method
Absorbance: 540nm
Features & Benefits
Simple procedure; takes less than 2 hours
Fast, sensitive and convenient
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
Fast, sensitive and convenient
This assay provides a convenient and non-radioactive alternative to carry out cytotoxicity assays. This assay relies on the ability of neutral cells to stain lysosomes of viable cells.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Neutral Red Cell Cytotoxicity assay kit
Neutral red cell cytotoxicity assay is one of the common methods used to detect cell viability or drug cytotoxicity. The principle of this assay is based on the detection of viable cells via the uptake of the dye neutral red. Neutral red is a eurhodin dye that stains lysosomes in viable cells. Viable cells can take up neutral red via active transport and incorporate the dye into their lysosomes but non-viable cells cannot not take up this chromophore. Consequently, after washing, viable cells can release the incorporated dye in under acidified-extracted conditions. The amount of released dye can be used to determine the total number of viable cells or drug cytotoxicity. The neutral red uptake assay provides a quantitative measurement of the number of viable cells and can be measured at OD 540 nm. Neutral Red Cell Cytotoxicity Assay Kit is simple, accurate, and reproducible. It also includes Doxorubicin, as a positive control. This kit offers an excellent and efficient method for in vitro cytotoxicity studies as well as high-throughput drug screening that can detect between 5,000-50,000 cells per well.
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Product Notes
The Mammalian Neutral Red Cell Cytotoxicity (Catalog #AAA849670) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849670 Assay Kit recognizes Mammalian Neutral Red Cell Cytotoxicity. It is sometimes possible for the material contained within the vial of "Neutral Red Cell Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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