Hexokinase 2 Active Protein | HXK2 active protein

Human Recombinant Hexokinase 2

Cat. Num. AAA844666

• Gene Names: HK2; HKII; HXK2
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Hexokinase 2; Human Recombinant Hexokinase 2; Hexokinase type II; Muscle form hexokinase; HK2.; HXK2 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR
• Sequence Length: 917

• Applicable Applications for HXK2 active protein: SDS-PAGE
• Biological Activity: Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP.
• Unit Definition: In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for HXK2 active protein

Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV This enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. It also constitutes the principal regulated isoform in many other cell types and is increased in many cancers. Although found in NIDDM patients, genetic variations of HK 2 do not contribute to the disease.

Product Categories/Family for HXK2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 15553127
• NCBI GeneID: 3099
• NCBI Accession #: NP_000180.2
• NCBI GenBank Nucleotide #: NM_000189.4
• UniProt Accession #: P52789; Q8WU87; Q9UN82; D6W5J2
• Molecular Weight: 104.1 kDa
• NCBI Official Full Name: hexokinase-2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2
• NCBI Protein Information: hexokinase-2
• UniProt Protein Name: Hexokinase-2
• Protein Family: Hexokinase
• UniProt Gene Name: HK2
• UniProt Synonym Gene Names: HK II
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.1

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; glucose metabolic process; pathogenesis; regulation of glucose import; glucose transport; transmembrane transport