Typical Testing Data/Standard Curve (for reference only)
Human Hexokinase 2 ELISA Kit | HK2 elisa kit
Human Hexokinase 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK2 antibody and an HK2-HRP conjugate. The assay sample and buffer are incubated together with HK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK2 concentration since HK2 from samples and HK2-HRP conjugate compete for the anti-HK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HK2 from the sample, fewer sites are left to bind HK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]
Uniprot Description
Catalytic activity: ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation: Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.
Pathway: Carbohydrate metabolism; hexose metabolism.
Subunit structure: Monomer
Subcellular location: Mitochondrion outer membrane
By similarity. Note: Its hydrophobic N-terminal sequence may be involved in membrane binding
Tissue specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
Domain: The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
Polymorphism: Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.
Miscellaneous: In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities: Belongs to the hexokinase family.
Research Articles on HK2
Similar Products
Product Notes
The Human HK2 hk2 (Catalog #AAA726852) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726852 ELISA Kit recognizes Human HK2. It is sometimes possible for the material contained within the vial of "Hexokinase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
