Mouse anti-Human HLA-G Monoclonal Antibody | anti-HLA-G antibody

HLA-G Antibody

Cat. Num. AAA9231793

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: HLA-G; Monoclonal Antibody; HLA-G Antibody; HLA-6.0; HLAG; HLA class I histocompatibility antigen; alpha chain G; HLA G antigen; MHC class I antigen G; anti-HLA-G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Purified His-tagged HLA-G protein was used to produced this monoclonal antibody.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 338

• Applicable Applications for anti-HLA-G antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Antigen Source: Human
• Antigen Region: 1-318
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from A549,Ramos,MDA-MB-231 cell line (from left to right), using HLA-G Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

Related Product Information for anti-HLA-G antibody

Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.

References

Shukla H., et al. Nucleic Acids Res. 18:2189-2189(1990). Geraghty D.E., et al. Proc. Natl. Acad. Sci. U.S.A. 84:9145-9149(1987). Ishitani A., et al. Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases. Hampe A., et al. DNA Seq. 10:263-299(1999). Shiina S., et al. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 122132
• NCBI GeneID: 3135
• UniProt Accession #: P17693
• Molecular Weight: 38224
• NCBI Official Full Name: HLA class I histocompatibility antigen, alpha chain G
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Synonym Gene Names: HLA-6.0; HLAG
• UniProt Entry Name: HLAG_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]