Rabbit Hexokinase Type II Polyclonal Antibody | anti-HK2 antibody

Hexokinase Type II (HXK II)

Cat. Num. AAA617799

• Gene Names: HK2; HKII; HXK2; DKFZp686M1669
• Reactivity: Human, Monkey
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Hexokinase Type II; Polyclonal Antibody; Hexokinase Type II (HXK II); Anti -Hexokinase Type II (HXK II); anti-HK2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human hexokinase (isoform II) protein at ~102kD. Species Crossreactivity: monkey
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-HK2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide corresponding to human hexokinase (isoform II).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of extracts from HeLa, SH-SY5Y and LN18 cells using MBS617799.)

Testing Data

Related Product Information for anti-HK2 antibody

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane, and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with the rapid cell growth and proliferation.

Product Categories/Family for anti-HK2 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 15553127
• NCBI GeneID: 3099
• NCBI Accession #: NP_000180.2
• NCBI GenBank Nucleotide #: NM_000189.4
• UniProt Accession #: P52789; Q8WU87; Q9UN82; D6W5J2; Q09LL6; Q53QX9; Q53SG7; Q68E10; Q7Z7Q6
• Molecular Weight: 102,380 Da
• NCBI Official Full Name: hexokinase-2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2; DKFZp686M1669
• NCBI Protein Information: hexokinase-2; HK II; OTTHUMP00000160433; OTTHUMP00000202738; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
• UniProt Protein Name: Hexokinase-2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK2
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Carbohydrate Metabolism - starch and sucrose; Mitochondrial; EC 2.7.1.1; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; glucose metabolic process; cell glucose homeostasis; pathogenesis; regulation of glucose import; glucose transport; transmembrane transport