Hexokinase 2 Active Protein | HXK2 active protein

Recombinant Hexokinase 2

Cat. Num. AAA201056

• Gene Names: HK2; HKII; HXK2
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Hexokinase 2; Recombinant Hexokinase 2; HKII; HXK2 active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; 20mM Tris-HCl Buffer (pH8.0) Containing 10% Glycerol, 0.1M NaCl, 0.1mM PMSF.
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 1-917aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR
• Sequence Length: 917

• Applicable Applications for HXK2 active protein: Enzyme Activity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Biological Activity: Specific activity is > 40,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for HXK2 active protein

Hexokinase2 is one of four highly homologous hexokinase isoforms in mammalian cells. Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for HXK2 active protein

Signal Transduction; Metabolism; Cancer

References

Travis SM. et al. (1997) FEBS Lett. 412(3):415-9. Brautigan D.L. et al. (2005) Biochemistry 44(33):11067-73.

NCBI and Uniprot Product Information

• NCBI GI #: 39963174
• NCBI GeneID: 3099
• UniProt Accession #: P52789
• Molecular Weight: 104.5 kDa (937aa)
• NCBI Official Full Name: Hexokinase 2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2
• NCBI Protein Information: hexokinase-2
• UniProt Protein Name: Hexokinase-2
• Protein Family: Hexokinase
• UniProt Gene Name: HK2
• UniProt Synonym Gene Names: HK II
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Kinase, other; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; cell glucose homeostasis; regulation of glucose import; pathogenesis; glucose transport; transmembrane transport