Rabbit anti-Human Hexokinase 2 Polyclonal Antibody | anti-HK2 antibody

Anti-Hexokinase 2 Antibody

Cat. Num. AAA847330

• Gene Names: HK2; HKII; HXK2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purified rabbit IgG
• Synonyms: Hexokinase 2; Polyclonal Antibody; Anti-Hexokinase 2 Antibody; Hexokinase type II; Muscle form hexokinase; HK2; anti-HK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Polyclonal Antibody to detect Hexokinase 2 in human samples
• Purity/Purification: Affinity purified rabbit IgG
• Form/Format: Colorless liquid. In PBS pH7.2, containing 30% glycerol, 0.5% BSA, 5mM EDTA and 0.03% Proclin

• Applicable Applications for anti-HK2 antibody: Western Blot (WB)
• Application Notes: WB: 1-4ug
• Immunogen: Human recombinant Hexokinase 2
• Positive Control: WB: Jurkat cell lysate, 3T3 cell lysate
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use.; Shelf life: ~12 months

Western Blot (WB)

Related Product Information for anti-HK2 antibody

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV This enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N terminal sequence capable of targeting the hexokinase to mitochondria. It also constitutes the principal regulated isoform in many other cell types and is increased in many cancers. Although found in NIDDM patients, genetic variations of HK 2 do not contribute to the disease.

Product Categories/Family for anti-HK2 antibody

Signal Transduction; Antibodies & Supporting Tools; Trial Size Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 39963174
• NCBI GeneID: 3099
• UniProt Accession #: P52789; Q8WU87; Q9UN82; D6W5J2
• Molecular Weight: 102,380 Da
• NCBI Official Full Name: Hexokinase 2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2
• NCBI Protein Information: hexokinase-2; HK II; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
• UniProt Protein Name: Hexokinase-2
• Protein Family: Probable histidine kinase
• UniProt Gene Name: HK2
• UniProt Synonym Gene Names: HK II
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Kinase, other; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; cell glucose homeostasis; regulation of glucose import; pathogenesis; glucose transport; transmembrane transport