Typical Testing Data/Standard Curve (for reference only)
Total Cholesterol and Cholesteryl EsterII Assay Kit
Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II
Synonyms
Total Cholesterol and Cholesteryl EsterII; Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II; Total Cholesterol and Cholesteryl EsterII assay kit
Samples
Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
Features & Benefits
Simple procedure; takes ~1 hour
Fast and convenient
Kit contains the necessary reagents for measurement of cholesterol in various biological samples
Fast and convenient
Kit contains the necessary reagents for measurement of cholesterol in various biological samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Total Cholesterol and Cholesteryl EsterII assay kit
Product Information: Cholesterol/Cholesteryl Ester Quantitation Kit II: Colorimetric Assay to Measure Total Cholesterol, Cholesteryl ester & Free Cholesterol in a variety of Biological Samples such as Tissue, Cells, Serum etc. within 40 min. Simple, Convenient & Reliable. 100 Assays
Background: Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Background: Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
Summary: Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Product Categories/Family for Total Cholesterol and Cholesteryl EsterII assay kit
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Product Notes
The Total Cholesterol and Cholesteryl EsterII (Catalog #AAA841615) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Total Cholesterol and Cholesteryl EsterII, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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