Western Blot (WB)
(Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit VASH1 Polyclonal Antibody | anti-VASH1 antibody
VASH1 Antibody
Gene Names
VASH1; KIAA1036
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
VASH1 Antibody is affinity chromatography purified via peptide column.
Synonyms
VASH1; Polyclonal Antibody; VASH1 Antibody; KIAA1036; VASH; Vasohibin-1; vasohibin 1; anti-VASH1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
VASH1 antibody is predicted to not cross-react with other vasohibin protein family members. At least two isoforms are known to exist; this antibody will recognize only the long isoform.
Purity/Purification
VASH1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
365
Applicable Applications for anti-VASH1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
VASH1 antibody can be used for detection of VASH1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
VASH1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human VASH1.
Buffer
VASH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
VASH1 antibody can be stored at 4 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of VASH1 in human brain tissue lysate with VASH1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of VASH1 in mouse brain tissue with VASH1 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of VASH1 in mouse brain tissue with VASH1 antibody at 20 μg/mL.)
Related Product Information for anti-VASH1 antibody
VASH1 Antibody: VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner. VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development. VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2). The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis. Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,845 Da
NCBI Official Full Name
vasohibin-1
NCBI Official Synonym Full Names
vasohibin 1
NCBI Official Symbol
VASH1
NCBI Official Synonym Symbols
KIAA1036
NCBI Protein Information
vasohibin-1
UniProt Protein Name
Vasohibin-1
Protein Family
UniProt Gene Name
VASH1
UniProt Synonym Gene Names
KIAA1036; VASH
UniProt Entry Name
VASH1_HUMAN
Uniprot Description
VASH1: Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity. Belongs to the vasohibin family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: extracellular space; apical part of cell; endoplasmic reticulum; cytoplasm
Biological Process: negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to wounding; angiogenesis; negative regulation of blood vessel endothelial cell migration; cell cycle arrest
Research Articles on VASH1
Similar Products
Product Notes
The VASH1 vash1 (Catalog #AAA150211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VASH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VASH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). VASH1 antibody can be used for detection of VASH1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the VASH1 vash1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VASH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
