Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using eIF2alpha(Phospho-Ser51) antibody)
Rabbit eIF2 alpha Polyclonal Antibody | anti-eIF2 alpha antibody
eIF2 alpha antibody
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
eIF2 alpha antibody was purified by immunoaffinity chromatography.
Synonyms
eIF2 alpha; Polyclonal Antibody; eIF2 alpha antibody; Polyclonal eIF2 alpha; Anti-eIF2 alpha; Phospho Ser51; eIF2; anti-eIF2 alpha antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
eIF2 alpha antibody was purified by immunoaffinity chromatography.
Form/Format
Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-eIF2 alpha antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC: 1:50-1:100
WB: 1:500-1:1000
IHC: 1:50-1:100
WB: 1:500-1:1000
Biological Significance
Eukaryotic Initiation Factor 2 is a eukaryotic initiation factor. It is required in the initiation of translation. In this fundamental process of life, the ribosome builds proteins according to the information encoded on the mRNA. eIF2 mediates the binding of tRNAmet to the ribosome in a GTP-dependent manner. eIF2 is a heterotrimer consisting of an alpha (also called subunit 1), a beta (subunit 2), and a gamma (subunit 3) subunit.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
eIF2 alpha antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2A as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using eIF2alpha(Phospho-Ser51) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using eIF2alpha(Phospho-Ser51) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from K562 cells untreated (Lane 1) or treated with IFN-alpha (Lane 2) using eIF2alpha (Phospho-Ser51) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from K562 cells untreated (Lane 1) or treated with IFN-alpha (Lane 2) using eIF2alpha (Phospho-Ser51) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser51) antibody (left) or the same antibody preincubated with with blocking peptide (right))
Related Product Information for anti-eIF2 alpha antibody
Rabbit polyclonal eIF2 alpha antibody
Product Categories/Family for anti-eIF2 alpha antibody
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Product Notes
The eIF2 alpha (Catalog #AAA835718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's eIF2 alpha can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100-1:200 IHC: 1:50-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the eIF2 alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF2 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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