Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
Rabbit TAP Polyclonal Antibody | anti-TAP antibody
TAP antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
TAP antibody was purified by Protein A chromatography
Synonyms
TAP; Polyclonal Antibody; TAP antibody; Polyclonal TAP; Anti-TAP; MEX67; DKFZp667O0311; NXF1; tip associating protein; nuclear RNA export factor 1; tip-associating protein; tip-associated protein; mRNA export factor TAP; anti-TAP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TAP antibody was purified by Protein A chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
10.00 mg/ml (varies by lot)
Sequence Length
105
Applicable Applications for anti-TAP antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
WB: 1:500-1:3000
Biological Significance
This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p.
Cross-Reactivity
Human, Mouse
Immunogen
TAP antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 449 and 619 of TAP
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5 % SDS PAGE gel and TAP antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP antibody at 1:200 dilution.)
Related Product Information for anti-TAP antibody
Affinity purified Rabbit polyclonal TAP antibody
Product Categories/Family for anti-TAP antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TAP tap (Catalog #AAA835674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TAP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the TAP tap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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