<
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TCP1 epsilon antibody at a dilution of 1:1000)
Rabbit TCP1 epsilon Polyclonal Antibody | anti-TCP1 epsilon antibody
TCP1 epsilon antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
TCP1 epsilon antibody was purified by antigen-affinity chromatography
Synonyms
TCP1 epsilon; Polyclonal Antibody; TCP1 epsilon antibody; Polyclonal TCP1 epsilon; Anti-TCP1 epsilon; CCT-epsilon; T-complex protein 1 epsilon subunit; TCP epsilon 1; CCT5; T-complex protein 1 subunit epsilon; TCP epsilon-1; TCP-1-epsilon; CCTE; KIAA0098; anti-TCP1 epsilon antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TCP1 epsilon antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
551
Applicable Applications for anti-TCP1 epsilon antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Biological Significance
This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex, also known as the TCP1 ring complex. This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TCP1 epsilon antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and TCP1 epsilon antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5 % SDS PAGE gel and TCP1 epsilon antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5 % SDS PAGE gel and TCP1 epsilon antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon antibody at 1:200 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using TCP1 epsilon antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded NCIN87 xenograft using TCP1 epsilon antibody at a dilution of 1:500)
Related Product Information for anti-TCP1 epsilon antibody
Affinity purified Rabbit polyclonal TCP1 epsilon antibody
Product Categories/Family for anti-TCP1 epsilon antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
60 kDa
NCBI Official Full Name
TCP1
UniProt Protein Name
T-complex protein 1 subunit epsilon
UniProt Gene Name
CCT5
UniProt Synonym Gene Names
TCP5; TCP-1-epsilon
UniProt Entry Name
TCPE_YEAST
Uniprot Description
Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.
Similar Products
Product Notes
The TCP1 epsilon cct5 (Catalog #AAA835064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TCP1 epsilon can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the TCP1 epsilon cct5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCP1 epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
