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Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and NULP1 antibody at a dilution of 1:1000)
Rabbit NULP1 Polyclonal Antibody | anti-NULP1 antibody
NULP1 antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
NULP1 antibody was purified by antigen-affinity chromatography
Synonyms
NULP1; Polyclonal Antibody; NULP1 antibody; Polyclonal NULP1; Anti-NULP1; hKIAA1049; Hulp1; transcription factor 25; PRO2620; TCF-25; NULP-1; NULP 1; KIAA1049; TCF25; nuclear localized protein 1; anti-NULP1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
NULP1 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
676
Applicable Applications for anti-NULP1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Biological Significance
TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development.
Cross-Reactivity
Human, Mouse
Immunogen
NULP1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 427 and 676 of NULP1
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and NULP1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 7.5 % SDS PAGE gel and NULP1 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and NULP1 antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5 % SDS PAGE gel and NULP1 antibody at a dilution of 1:500)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NULP1 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NULP1 antibody at 1:200 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded FaDu xenograft using NULP1 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded FaDu xenograft using NULP1 antibody at a dilution of 1:100)
Related Product Information for anti-NULP1 antibody
Affinity purified Rabbit polyclonal NULP1 antibody
Product Categories/Family for anti-NULP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
77 kDa
NCBI Official Full Name
NULP1
UniProt Protein Name
Transcription factor 25
UniProt Gene Name
TCF25
UniProt Synonym Gene Names
KIAA1049; NULP1; TCF-25
UniProt Entry Name
TCF25_HUMAN
Uniprot Description
TCF25: May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development. Belongs to the TCF25 family.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 16q24.3
Cellular Component: nucleus
Molecular Function: protein binding; DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent; heart development; negative regulation of transcription from RNA polymerase II promoter
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Product Notes
The NULP1 tcf25 (Catalog #AAA837529) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NULP1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the NULP1 tcf25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NULP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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