Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded lung CA patient tumor using RPA14 antibody at a dilution of 1:100)
Rabbit RPA14 Polyclonal Antibody | anti-RPA14 antibody
RPA14 antibody
Applications
Immunohistochemistry, Western Blot
Purity
RPA14 antibody was purified by antigen-affinity chromatography
Synonyms
RPA14; Polyclonal Antibody; RPA14 antibody; Polyclonal RPA14; Anti-RPA14; REPA3; RPA 14; RPA-14; replication protein A 14 kDa subunit; replication factor A protein 3; RPA3; RF-A protein 3; RP-A p14; anti-RPA14 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
RPA14 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
122
Applicable Applications for anti-RPA14 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:250
WB: 1:500-1:3000
WB: 1:500-1:3000
Biological Significance
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Cross-Reactivity
Human
Immunogen
RPA14 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 121 of RPA14
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded lung CA patient tumor using RPA14 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded lung CA patient tumor using RPA14 antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 15 % SDS PAGE gel and RPA14 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:HeLa S3; B:Hep G2; C:MOLT4) using a 15 % SDS PAGE gel and RPA14 antibody at a dilution of 1:1000)
Related Product Information for anti-RPA14 antibody
Affinity purified Rabbit polyclonal RPA14 antibody
Product Categories/Family for anti-RPA14 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
14 kDa
NCBI Official Full Name
RPA14
UniProt Protein Name
RPA14
Protein Family
UniProt Gene Name
rpa2
UniProt Entry Name
Q977W8_9EURY
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Product Notes
The RPA14 rpa2 (Catalog #AAA835439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RPA14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:250 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the RPA14 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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