Typical Testing Data/Standard Curve (for reference only)
Mouse Macrophage Inflammatory Protein 1a ELISA Kit | MIP1a elisa kit
Mouse Macrophage Inflammatory Protein 1a ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This MIP1A/CCL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MIP1A/CCL3. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY MIP1A/CCL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP1A/CCL3 antibody and an MIP1A/CCL3-HRP conjugate. The assay sample and buffer are incubated together with MIP1A/CCL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP1A/CCL3 concentration since MIP1A/CCL3 from samples and MIP1A/CCL3-HRP conjugate compete for the anti-MIP1A/CCL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP1A/CCL3 from the sample, fewer sites are left to bind MIP1A/CCL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1A/CCL3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
Uniprot Description
CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted, signal peptide; Chemokine; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol
Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; phospholipase activator activity; chemoattractant activity; protein kinase activity; CCR5 chemokine receptor binding
Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; response to toxin; behavior; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; negative regulation of bone mineralization; monocyte chemotaxis; regulation of cell shape; positive chemotaxis; cell-cell signaling; calcium ion transport; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; MAPKKK cascade; calcium-mediated signaling; cytoskeleton organization and biogenesis; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; positive regulation of tumor necrosis factor production; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration
Disease: Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on MIP1a
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Product Notes
The Mouse MIP1a ccl3 (Catalog #AAA730641) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730641 ELISA Kit recognizes Mouse MIP1a. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein 1a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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