Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant TUBA3E protein (red) or empty vector (blue) stained using TUBA3E antibody)
Mouse TUBA3E Monoclonal Antibody | anti-TUBA3E antibody
TUBA3E antibody
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
TUBA3E antibody was purified by affinity chromatography.
Synonyms
TUBA3E; Monoclonal Antibody; TUBA3E antibody; Monoclonal TUBA3E; Anti-TUBA3E; Tubulin alpha 3e; Alpha tubulin 3E; Tubulin alpha-3E chain; TUBAE 3; TUBAE-3; anti-TUBA3E antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
10B4
Purity/Purification
TUBA3E antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
450
Applicable Applications for anti-TUBA3E antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
FC: 1:100
WB: 1:2000
WB: 1:2000
Biological Significance
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Cross-Reactivity
Human
Immunogen
TUBA3E antibody was raised in mouse using a full length recombinant protein of human TUBA3E (NP_997195) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant TUBA3E protein (red) or empty vector (blue) stained using TUBA3E antibody)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant TUBA3E protein (red) or empty vector (blue) stained using TUBA3E antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBA3E protein (Right) or empty vector (Left) detected with TUBA3E antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBA3E protein (Right) or empty vector (Left) detected with TUBA3E antibody)
Related Product Information for anti-TUBA3E antibody
Mouse monoclonal TUBA3E antibody
Product Categories/Family for anti-TUBA3E antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,859 Da
NCBI Official Full Name
tubulin alpha-3E chain
NCBI Official Synonym Full Names
tubulin, alpha 3e
NCBI Official Symbol
TUBA3E
NCBI Protein Information
tubulin alpha-3E chain
UniProt Protein Name
Tubulin alpha-3E chain
Protein Family
UniProt Gene Name
TUBA3E
UniProt Entry Name
TBA3E_HUMAN
Uniprot Description
TUBA3E: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 2q21.1
Cellular Component: microtubule; cytoplasm; nucleus
Molecular Function: GTPase activity; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; metabolic process; microtubule-based process
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Product Notes
The TUBA3E tuba3e (Catalog #AAA834628) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TUBA3E can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). FC: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the TUBA3E tuba3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBA3E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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