Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal liver tissue, Primary antibody: 820728(MGEA5 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)
Rabbit anti-Human MGEA5/OGA Polyclonal Antibody | anti-MGEA5/OGA antibody
MGEA5/OGA Rabbit pAb
IHC: 1:25-1:100
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal liver tissue, Primary antibody: 820728(MGEA5 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)
Western Blot (WB)
(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal liver tissue, Primary antibody: 820728(MGEA5 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 820728(MGEA5 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 820728(MGEA5 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 820728(MGEA5 Antibody) at dilution 1/20. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 820728(MGEA5 Antibody) at dilution 1/20. (Original magnification: ×200))
NCBI and Uniprot Product Information
NCBI Description
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008]
Uniprot Description
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
Research Articles on MGEA5/OGA
Similar Products
Product Notes
The MGEA5/OGA mgea5 (Catalog #AAA4757804) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGEA5/OGA Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5/OGA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the MGEA5/OGA mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGEA5/OGA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
