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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using TUBB4 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Mouse TUBB4 Monoclonal Antibody | anti-TUBB4 antibody

TUBB4 antibody

Gene Names
TUBB4A; DYT4; TUBB4; beta-5
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
TUBB4 antibody was purified by affinity chromatography.
Synonyms
TUBB4; Monoclonal Antibody; TUBB4 antibody; Monoclonal TUBB4; Anti-TUBB4; Beta Tubulin 4; TUBB5; beta-5; TUBB 4; TUBB-4; anti-TUBB4 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
5C1
Purity/Purification
TUBB4 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-TUBB4 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Cross-Reactivity
Human
Immunogen
TUBB4 antibody was raised in mouse using a full length recombinant protein of human TUBB4 (NP_006078) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using TUBB4 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using TUBB4 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBB4 protein (Right) or empty vector (Left) detected with TUBB4 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TUBB4 protein (Right) or empty vector (Left) detected with TUBB4 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of TUBB4 protein in paraffin embedded Adenocarcinoma of Human colon tissue using TUBB4 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of TUBB4 protein in paraffin embedded Adenocarcinoma of Human colon tissue using TUBB4 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant TUBB4 protein using TUBB4 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant TUBB4 protein using TUBB4 antibody)
Related Product Information for anti-TUBB4 antibody
Mouse monoclonal TUBB4 antibody
Product Categories/Family for anti-TUBB4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,586 Da
NCBI Official Full Name
tubulin beta-4A chain isoform 3
NCBI Official Synonym Full Names
tubulin, beta 4A class IVa
NCBI Official Symbol
TUBB4A
NCBI Official Synonym Symbols
DYT4; TUBB4; beta-5
NCBI Protein Information
tubulin beta-4A chain
UniProt Protein Name
Tubulin beta-4A chain
Protein Family
UniProt Gene Name
TUBB4A
UniProt Synonym Gene Names
TUBB4; TUBB5
UniProt Entry Name
TBB4A_HUMAN

NCBI Description

This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]

Uniprot Description

TUBB4: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: internode region of axon; microtubule; cell soma; cytosol; nucleus; myelin sheath; cilium

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; transmembrane transport; microtubule-based process

Disease: Dystonia 4, Torsion, Autosomal Dominant

Research Articles on TUBB4

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Product Notes

The TUBB4 tubb4a (Catalog #AAA833460) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TUBB4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the TUBB4 tubb4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBB4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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