Mouse NMT2 Monoclonal Antibody | anti-NMT2 antibody

NMT2 antibody

Cat. Num. AAA832511

• Applications: Western Blot
• Purity: NMT2 antibody was purified by affinity chromatography.
• Synonyms: NMT2; Monoclonal Antibody; NMT2 antibody; Monoclonal NMT2; Anti-NMT2; NMT; NMT 2; NMT-2; anti-NMT2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1H7
• Purity/Purification: NMT2 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 63

• Applicable Applications for anti-NMT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:2000
• Biological Significance: N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases.
• Cross-Reactivity: Human
• Immunogen: NMT2 antibody was raised in Mouse using full length human recombinant protein of human NMT2 produced in HEK293T cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NMT2 protein (Right) or empty vector (Left) detected with NMT2 antibody)

Related Product Information for anti-NMT2 antibody

Mouse monoclonal NMT2 antibody

Product Categories/Family for anti-NMT2 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 13528864
• NCBI GeneID: 9397
• UniProt Accession #: O60551; Q53Y38; Q5VUC8; Q9BRB4; B0YJ49
• Molecular Weight: 56,980 Da
• NCBI Official Full Name: NMT2 protein, partial
• NCBI Official Synonym Full Names: N-myristoyltransferase 2
• NCBI Official Symbol: NMT2
• NCBI Protein Information: glycylpeptide N-tetradecanoyltransferase 2
• UniProt Protein Name: Glycylpeptide N-tetradecanoyltransferase 2
• Protein Family: Glycylpeptide N-tetradecanoyltransferase
• UniProt Gene Name: NMT2
• UniProt Synonym Gene Names: NMT 2
• UniProt Entry Name: NMT2_HUMAN

NCBI Description

This gene encodes one of two N-myristoyltransferase proteins. N-terminal myristoylation is a lipid modification that is involved in regulating the function and localization of signaling proteins. The encoded protein catalyzes the addition of a myristoyl group to the N-terminal glycine residue of many signaling proteins, including the human immunodeficiency virus type 1 (HIV-1) proteins, Gag and Nef. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

NMT2: Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins. Belongs to the NMT family.

Protein type: Transferase; EC 2.3.1.97

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: Golgi apparatus; extrinsic to membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: glycylpeptide N-tetradecanoyltransferase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; viral reproduction; viral protein processing; N-terminal peptidyl-glycine N-myristoylation; virus assembly