Rabbit anti-Human, Mouse MSC Polyclonal Antibody | anti-MSC antibody

MSC, CT (MSC, ABF1, BHLHA22, Musculin, Activated B Cell factor 1, Class A basic helix-loop-helix protein 22) (Biotin)

Cat. Num. AAA6322463

• Gene Names: MSC; ABF1; MYOR; ABF-1; bHLHa22
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MSC; Polyclonal Antibody; CT (MSC; ABF1; BHLHA22; Musculin; Activated B Cell factor 1; Class A basic helix-loop-helix protein 22) (Biotin); Activated B-cell factor 1; Class A basic helix-loop-helix protein 22; anti-MSC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MSC antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the C-terminal region of human MSC.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MSC Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MSC antibody detected the MSC protein (arrow).)

Related Product Information for anti-MSC antibody

The protein encoded by this gene is a transcriptional repressor capable of binding an E-box element either as a homodimer or as a heterodimer with E2A in vitro. The encoded protein also forms heterodimers with E2A proteins in vivo. This protein is capable of inhibiting the transactivation capability of E47, an E2A protein, in mammalian cells. This gene is a downstream target of the B-cell receptor signal transduction pathway. [provided by RefSeq].

Product Categories/Family for anti-MSC antibody

Antibodies; Signal Transduction

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Ushmorov, A., et al. Leukemia 22(10):1942-1944(2008). Knight, J.C., et al. Nat. Genet. 36(4):394-399(2004). Wong, J., et al. DNA Cell Biol. 20(8):465-471(2001). Robb, L., et al. Genomics 57(2):318-319(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 116805332
• NCBI GeneID: 9242
• NCBI Accession #: NP_005089.2
• NCBI GenBank Nucleotide #: NM_005098.3
• UniProt Accession #: O60682; O75946; Q53XZ2; Q9BRE7
• Molecular Weight: 22,068 Da
• NCBI Official Full Name: musculin
• NCBI Official Synonym Full Names: musculin
• NCBI Official Symbol: MSC
• NCBI Official Synonym Symbols: ABF1; MYOR; ABF-1; bHLHa22
• NCBI Protein Information: musculin; activated B-cell factor-1; class A basic helix-loop-helix protein 22; activated B-cell factor 1, homolog of mouse musculin
• UniProt Protein Name: Musculin
• Protein Family: Musculin
• UniProt Gene Name: MSC
• UniProt Synonym Gene Names: ABF1; BHLHA22; ABF-1; bHLHa22
• UniProt Entry Name: MUSC_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional repressor capable of binding an E-box element either as a homodimer or as a heterodimer with E2A in vitro. The encoded protein also forms heterodimers with E2A proteins in vivo. This protein is capable of inhibiting the transactivation capability of E47, an E2A protein, in mammalian cells. This gene is a downstream target of the B-cell receptor signal transduction pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

MSC: Transcription repressor capable of inhibiting the transactivation capability of TCF3/E47. May play a role in regulating antigen-dependent B-cell differentiation.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleus

Molecular Function: protein dimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; branchiomeric skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; palate development