FLT3 sirna

FLT3 siRNA (Mouse)

Cat. Num. AAA828723

• Gene Names: Flt3; Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FLT3; FLT3 siRNA (Mouse); FLK-2; FLT-3; Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase 2; Fms-like tyrosine kinase 3; Tyrosine-protein kinase receptor flk-2; CD antigen CD135; FLT3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FLT3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1000

• Applicable Applications for FLT3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FLT3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FLT3 sirna

siRNA to inhibit FLT3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 122937353
• NCBI GeneID: 14255
• NCBI Accession #: NP_034359.2
• NCBI GenBank Nucleotide #: NM_010229.2
• UniProt Accession #: Q00342
• Molecular Weight: 113,496 Da
• NCBI Official Full Name: receptor-type tyrosine-protein kinase FLT3
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 3
• NCBI Official Symbol: Flt3
• NCBI Official Synonym Symbols: Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04
• NCBI Protein Information: receptor-type tyrosine-protein kinase FLT3
• UniProt Protein Name: Receptor-type tyrosine-protein kinase FLT3
• Protein Family: Flt3 receptor-interacting lectin
• UniProt Gene Name: Flt3
• UniProt Synonym Gene Names: Flk-2; Flt-3; FLK-2; FLT-3
• UniProt Entry Name: FLT3_MOUSE

Uniprot Description

FLT3: a receptor tyrosine kinase of the PDGFR family that binds the FL cytokine. FLT3 -/- mice have an impaired developmental capacity of primitive hematopoietic progenitor cells of all lineages with the greatest impact on lymphopoietic precursors. Activating mutations found in one third of cases of acute myeloid leukemia (AML), as well as in acute lymphoblastic leukemia, acute promyelocytic leukemia and myelodysplastic syndrome. Inhibitors: Sutent and PKC412.

Protein type: Membrane protein, integral; Kinase, protein; Oncoprotein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; TK group; PDGFR family

Cellular Component: protein complex; cell surface; membrane; endoplasmic reticulum; cytoplasm; integral to membrane; plasma membrane; nucleus; cytosol; external side of plasma membrane

Molecular Function: transferase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein-tyrosine kinase activity; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; glucocorticoid receptor binding; protein kinase activity

Biological Process: negative regulation of B cell differentiation; pro-B cell differentiation; lymphocyte proliferation; peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; lymphoid progenitor cell differentiation; protein amino acid phosphorylation; negative regulation of cell proliferation; lymphocyte differentiation; myeloid progenitor cell differentiation; B cell differentiation; hemopoiesis; pro-T cell differentiation; positive regulation of protein amino acid phosphorylation; cell differentiation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; leukocyte homeostasis