RGCC sirna

RGCC siRNA (Human)

Cat. Num. AAA827652

• Gene Names: RGCC; RGC32; RGC-32; C13orf15; bA157L14.2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RGCC; RGCC siRNA (Human); C13orf15; RGC32; Regulator of cell cycle RGCC; Response gene to complement 32 protein; RGC-32; RGCC sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RGCC siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 137

• Applicable Applications for RGCC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RGCC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RGCC sirna

siRNA to inhibit RGCC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 132626811
• NCBI GeneID: 28984
• NCBI Accession #: NP_054778.2
• NCBI GenBank Nucleotide #: NM_014059.2
• UniProt Accession #: Q9H4X1; Q6NZ48; Q9UL69
• Molecular Weight: 12,924 Da
• NCBI Official Full Name: regulator of cell cycle RGCC
• NCBI Official Synonym Full Names: regulator of cell cycle
• NCBI Official Symbol: RGCC
• NCBI Official Synonym Symbols: RGC32; RGC-32; C13orf15; bA157L14.2
• NCBI Protein Information: regulator of cell cycle RGCC
• UniProt Protein Name: Regulator of cell cycle RGCC
• Protein Family: Regulator of cell cycle
• UniProt Gene Name: RGCC
• UniProt Synonym Gene Names: C13orf15; RGC32; RGC-32
• UniProt Entry Name: RGCC_HUMAN

NCBI Description

This gene is thought to regulate cell cycle progression. It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. The encoded protein localizes to the cytoplasm during interphase and to centrosomes during mitosis. The protein forms a complex with polo-like kinase 1. The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

RGC32: modulates the activity of cell cycle-specific kinases. Interacts with CDK1 and PLK1. Enhances CDK1 activity and contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation. Interacts with SMAD3. Cytoplasmic in unstimulated cells; translocates to the nucleus following activation by complement. Associated with the centrosome during prometaphase and metaphase. Induced by Epstein-Barr virus (EBV). Up-regulated in aorta endothelial cells in response to complement activation. Important in vascular smooth muscle cell (VSMC) differentiation following transforming growth factor-beta (TGF-beta) induction. Its expression is greatly induced by TGF-beta in neural crest cells, leading to the induction of VSMC gene promoters. Smad and RhoA signaling are important for RGC-32 activation in neural crest cells. Forms a complex with hnRNP A/B that regulates the transcription of VSMC differentiation genes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase activator activity; protein kinase binding

Biological Process: positive regulation of mitosis; positive regulation of collagen biosynthetic process; negative regulation of cytokine secretion; negative regulation of blood vessel endothelial cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; complement activation; negative regulation of cell proliferation; negative regulation of angiogenesis; negative regulation of exit from mitosis; positive regulation of stress fiber formation; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cytokine secretion