Response Gene To Complement 32 Recombinant Protein | RGC32 recombinant protein

Recombinant Response Gene To Complement 32 (RGC32)

Cat. Num. AAA2029251

• Gene Names: RGCC; RGC32; RGC-32; C13orf15; bA157L14.2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Response Gene To Complement 32; Recombinant Response Gene To Complement 32 (RGC32); RGC32 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MKPPAAQGSP AAAAAAAPAL DSAAAEDLSD ALCEFDAVLA DFASPFHERH FHYEEHLERM KRRSSASVSD SSGFSDSESA DSLYRNSFSF SDEKLNSPTD STPALLSATV TPQKAKLGDTKELEAFIADL DKTLASM
• Sequence Length: 78

• Applicable Applications for RGC32 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 18.3kDa
• Accurate Molecular Mass (KD): 18kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Met137
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119629069
• NCBI GeneID: 28984
• Molecular Weight: 12,924 Da
• NCBI Official Full Name: response gene to complement 32, isoform CRA_b
• NCBI Official Synonym Full Names: regulator of cell cycle
• NCBI Official Symbol: RGCC
• NCBI Official Synonym Symbols: RGC32; RGC-32; C13orf15; bA157L14.2
• NCBI Protein Information: regulator of cell cycle RGCC
• UniProt Protein Name: Regulator of cell cycle RGCC
• UniProt Gene Name: RGCC
• UniProt Synonym Gene Names: C13orf15; RGC32; RGC-32
• UniProt Entry Name: RGCC_HUMAN

NCBI Description

This gene is thought to regulate cell cycle progression. It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. The encoded protein localizes to the cytoplasm during interphase and to centrosomes during mitosis. The protein forms a complex with polo-like kinase 1. The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

RGC32: modulates the activity of cell cycle-specific kinases. Interacts with CDK1 and PLK1. Enhances CDK1 activity and contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation. Interacts with SMAD3. Cytoplasmic in unstimulated cells; translocates to the nucleus following activation by complement. Associated with the centrosome during prometaphase and metaphase. Induced by Epstein-Barr virus (EBV). Up-regulated in aorta endothelial cells in response to complement activation. Important in vascular smooth muscle cell (VSMC) differentiation following transforming growth factor-beta (TGF-beta) induction. Its expression is greatly induced by TGF-beta in neural crest cells, leading to the induction of VSMC gene promoters. Smad and RhoA signaling are important for RGC-32 activation in neural crest cells. Forms a complex with hnRNP A/B that regulates the transcription of VSMC differentiation genes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase activator activity; protein kinase binding

Biological Process: positive regulation of mitosis; negative regulation of cytokine secretion; positive regulation of collagen biosynthetic process; negative regulation of blood vessel endothelial cell migration; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; complement activation; negative regulation of cell proliferation; negative regulation of angiogenesis; negative regulation of exit from mitosis; negative regulation of endothelial cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cytokine secretion