Human Response Gene To Complement 32 ELISA Kit | RGC32 elisa kit

Human RGC32 (Response Gene To Complement 32) ELISA Kit

Cat. Num. AAA2533351

• Gene Names: RGCC; RGC32; RGC-32; C13orf15; bA157L14.2
• Reactivity: Human
• Synonyms: Response Gene To Complement 32; Human RGC32 (Response Gene To Complement 32) ELISA Kit; RGC32 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human RGC32 in samples. No significant cross-reactivity or interference between Human RGC32 and analogues was observed.
• Sequence Length: 78

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RGC32 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RGC32 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RGC32 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RGC32 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RGC32. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RGC32 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RGC32, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RGC32. You can calculate the concentration of Human RGC32 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119629069
• NCBI GeneID: 28984
• Molecular Weight: 12,924 Da
• NCBI Official Full Name: response gene to complement 32, isoform CRA_b
• NCBI Official Synonym Full Names: regulator of cell cycle
• NCBI Official Symbol: RGCC
• NCBI Official Synonym Symbols: RGC32; RGC-32; C13orf15; bA157L14.2
• NCBI Protein Information: regulator of cell cycle RGCC
• UniProt Protein Name: Regulator of cell cycle RGCC
• UniProt Gene Name: RGCC
• UniProt Synonym Gene Names: C13orf15; RGC32; RGC-32
• UniProt Entry Name: RGCC_HUMAN

NCBI Description

This gene is thought to regulate cell cycle progression. It is induced by p53 in response to DNA damage, or by sublytic levels of complement system proteins that result in activation of the cell cycle. The encoded protein localizes to the cytoplasm during interphase and to centrosomes during mitosis. The protein forms a complex with polo-like kinase 1. The protein also translocates to the nucleus in response to treatment with complement system proteins, and can associate with and increase the kinase activity of cell division cycle 2 protein. In different assays and cell types, overexpression of this protein has been shown to activate or suppress cell cycle progression. [provided by RefSeq, Jul 2008]

Uniprot Description

RGC32: modulates the activity of cell cycle-specific kinases. Interacts with CDK1 and PLK1. Enhances CDK1 activity and contribute to the regulation of the cell cycle. May inhibit growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. Fibrogenic factor contributing to the pathogenesis of renal fibrosis through fibroblast activation. Interacts with SMAD3. Cytoplasmic in unstimulated cells; translocates to the nucleus following activation by complement. Associated with the centrosome during prometaphase and metaphase. Induced by Epstein-Barr virus (EBV). Up-regulated in aorta endothelial cells in response to complement activation. Important in vascular smooth muscle cell (VSMC) differentiation following transforming growth factor-beta (TGF-beta) induction. Its expression is greatly induced by TGF-beta in neural crest cells, leading to the induction of VSMC gene promoters. Smad and RhoA signaling are important for RGC-32 activation in neural crest cells. Forms a complex with hnRNP A/B that regulates the transcription of VSMC differentiation genes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Cell cycle regulation

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: protein binding; protein kinase activator activity; protein kinase binding

Biological Process: activation of protein kinase activity; complement activation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; negative regulation of angiogenesis; negative regulation of blood vessel endothelial cell migration; negative regulation of cell proliferation; negative regulation of cytokine secretion; negative regulation of endothelial cell proliferation; negative regulation of exit from mitosis; positive regulation of collagen biosynthetic process; positive regulation of cytokine secretion; positive regulation of mitosis; positive regulation of stress fiber formation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter