Chicken Aggrecan ELISA Kit | AGC elisa kit

Chicken Aggrecan ELISA Kit

Cat. Num. AAA739153

• Gene Names: Acan; Agc; Agc1
• Reactivity: Chicken
• Synonyms: Aggrecan; Chicken Aggrecan ELISA Kit; AGC elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken AGC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AGC elisa kit

Principle of the Assay: anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AGC elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 11990616
• NCBI GeneID: 58968
• NCBI Accession #: NP_071526.1
• NCBI GenBank Nucleotide #: NM_022190.1
• Molecular Weight: 225,171 Da
• NCBI Official Full Name: aggrecan core protein
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: Acan
• NCBI Official Synonym Symbols: Agc; Agc1
• NCBI Protein Information: aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: Acan
• UniProt Synonym Gene Names: Agc; Agc1; CSPCP
• UniProt Entry Name: PGCA_RAT

NCBI Description

an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]

Uniprot Description

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.