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Typical Testing Data/Standard Curve

Mouse IL-10 ELISA Kit | IL10 elisa kit

Mouse IL-10 ELISA Kit

Gene Names
Il10; CSIF; Il-10
Reactivity
Mouse
Applications
ELISA
Synonyms
IL-10; Mouse IL-10 ELISA Kit; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF; IL10 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
The Mouse IL-10 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-10 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
178
Applicable Applications for IL10 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
Sensitivity
7 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Typical Testing Data/Standard Curve
Related Product Information for IL10 elisa kit
Principle of the Assay||The Mouse IL-10 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-10 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
References
Bathing effects of east saline groundwater concentrates on allergic (atopic) dermatitis-like skin lesions induced by 2,4-dinitrochlorobenzene in hairless mice. Choong?Gon Kim, Ji?Eun Lee, Da?Geum Jeong, Youn?Ho Lee, Sang?In Park, Dae?Geon Lee, Chang?Hyun Han, Su?Jin Kang, Chang?Hyun Song, Seong?Hun Choi, Young?Joon Lee, Sae?Kwang Ku (2017)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
20,641 Da
NCBI Official Full Name
Interleukin-10
NCBI Official Synonym Full Names
interleukin 10
NCBI Official Symbol
Il10
NCBI Official Synonym Symbols
CSIF; Il-10
NCBI Protein Information
interleukin-10
UniProt Protein Name
Interleukin-10
Protein Family
UniProt Gene Name
Il10
UniProt Synonym Gene Names
Il-10; IL-10; CSIF
UniProt Entry Name
IL10_MOUSE

NCBI Description

This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, Sep 2015]

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted; Secreted, signal peptide

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; interleukin-10 receptor binding

Biological Process: defense response to bacterium; defense response to protozoan; immune response; inflammatory response; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; negative regulation of cytokine production; negative regulation of cytokine secretion during immune response; negative regulation of inflammatory response; negative regulation of interferon-gamma production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of membrane protein ectodomain proteolysis; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of tumor necrosis factor biosynthetic process; negative regulation of tumor necrosis factor production; positive regulation of B cell apoptosis; positive regulation of cytokine secretion; positive regulation of JAK-STAT cascade; positive regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; receptor biosynthetic process; regulation of gene expression; regulation of sensory perception of pain; response to glucocorticoid stimulus; response to molecule of bacterial origin

Research Articles on IL10

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Product Notes

The Mouse IL10 il10 (Catalog #AAA8244590) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244590 ELISA Kit recognizes Mouse IL10. AAA Biotech's IL-10 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IL10 il10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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