DAB2IP sirna

DAB2IP siRNA (Human)

Cat. Num. AAA8230515

• Gene Names: DAB2IP; AIP1; AIP-1; AF9Q34; DIP1/2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAB2IP; DAB2IP siRNA (Human); AF9Q34; AIP1; KIAA1743; Disabled homolog 2-interacting protein; DAB2 interaction protein; DAB2-interacting protein; ASK-interacting protein 1; AIP-1; DOC-2/DAB-2 interactive protein; DAB2IP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DAB2IP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1132

• Applicable Applications for DAB2IP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DAB2IP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAB2IP sirna

siRNA to inhibit DAB2IP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 94967023
• NCBI GeneID: 153090
• NCBI Accession #: NP_115941.2
• NCBI GenBank Nucleotide #: NM_032552.3
• UniProt Accession #: Q5VWQ8; Q8TDL2; Q96SE1; Q9C0C0; A6H8V2; A6NHI9; B0QZB1; G3XA90
• Molecular Weight: 125,041 Da
• NCBI Official Full Name: disabled homolog 2-interacting protein isoform 1
• NCBI Official Synonym Full Names: DAB2 interacting protein
• NCBI Official Symbol: DAB2IP
• NCBI Official Synonym Symbols: AIP1; AIP-1; AF9Q34; DIP1/2
• NCBI Protein Information: disabled homolog 2-interacting protein
• UniProt Protein Name: Disabled homolog 2-interacting protein
• Protein Family: Disabled homolog 2-interacting protein
• UniProt Gene Name: DAB2IP
• UniProt Synonym Gene Names: AF9Q34; AIP1; KIAA1743; DAB2 interaction protein; DAB2-interacting protein; AIP-1
• UniProt Entry Name: DAB2P_HUMAN

NCBI Description

DAB2IP is a Ras (MIM 190020) GTPase-activating protein (GAP) that acts as a tumor suppressor. The DAB2IP gene is inactivated by methylation in prostate and breast cancers (Yano et al., 2005 [PubMed 15386433]).[supplied by OMIM, May 2010]

Uniprot Description

DAB2IP: Functions as a Ras GTPase-activating protein. Through its interaction with MAP3K5 disrupts the association of MAP3K5 with the inhibitory 14-3-3 complex. Mediates TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK- NF-kappa-B signaling. May act as a tumor suppressor gene. A chromosomal aberration involving DAB2IP is found in a patient with acute myeloid leukemia (AML). Translocation t(9;11)(q34;q23) with MLL. May give rise to a MLL-DAB2IP fusion protein lacking the PH domain. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Ras; GAPs; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q33.1-q33.3

Cellular Component: cell soma; endocytic vesicle; axon; dendrite; cytoplasm; plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity; protein phosphatase 2A binding; mitogen-activated protein kinase kinase binding; death receptor binding; mitogen-activated protein kinase kinase kinase binding; protein kinase binding; protein binding; vascular endothelial growth factor receptor 2 binding; protein complex binding; phosphoinositide 3-kinase binding; SH3 domain binding; kinase binding; phosphatidylinositol 3-phosphate binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of JNK activity; negative regulation of MAP kinase activity; regulation of protein heterodimerization activity; positive regulation of apoptosis; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; activation of JNK activity; negative regulation of cell proliferation; negative regulation of phosphoinositide 3-kinase activity; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of growth; regulation of protein complex assembly; negative regulation of Ras protein signal transduction; angiogenesis; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; layer formation in the cerebral cortex; negative regulation of epithelial cell proliferation; negative regulation of vascular endothelial growth factor receptor signaling pathway; transformed cell apoptosis; lumen formation; negative regulation of I-kappaB kinase/NF-kappaB cascade; cell cycle; negative regulation of angiogenesis; unfolded protein response, activation of signaling protein activity; induction of apoptosis via death domain receptors; inhibition of NF-kappaB transcription factor; cellular protein catabolic process; negative regulation of fibroblast proliferation; negative regulation of toll-like receptor 4 signaling pathway; activation of MAPKKK activity; positive regulation of protein catabolic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; I-kappaB phosphorylation; cell locomotion involved in cerebral cortex radial glia guided migration; neurite morphogenesis; negative regulation of phosphoinositide 3-kinase cascade