SDS-Page
Collagen Type VIII Alpha 1 (COL8a1) Recombinant Protein | COL8a1 recombinant protein
Recombinant Collagen Type VIII Alpha 1 (COL8a1)
Gene Names
Col8a1; Col8a-1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Collagen Type VIII Alpha 1 (COL8a1); Recombinant Collagen Type VIII Alpha 1 (COL8a1); COL8a1 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Concentration
Original Concentration: 200 ug/mL (varies by lot)
Applicable Applications for COL8a1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism Species
Mus musculus (Mouse)
Source
Prokaryotic expression
Residues
Glu583~Pro743
Tags
N-terminal His Tag
Traits
Freeze-dried powder
Buffer formulation
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Predicted isoelectric point
6.6
Predicted Molecular Mass
19.0kDa
Accurate Molecular Mass
18kDa as determined by SDS-PAGE reducing conditions.
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.05-1.0 mg/mL. Do not
vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
collagen alpha-1(VIII) chain
NCBI Official Synonym Full Names
collagen, type VIII, alpha 1
NCBI Official Symbol
Col8a1
NCBI Official Synonym Symbols
Col8a-1
NCBI Protein Information
collagen alpha-1(VIII) chain; collagen alpha-1(VIII) chain; procollagen type VIII alpha 1; procollagen, type VIII, alpha 1
UniProt Protein Name
Collagen alpha-1(VIII) chain
Protein Family
UniProt Gene Name
Col8a1
UniProt Entry Name
CO8A1_MOUSE
Uniprot Description
COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; intracellular membrane-bound organelle; extracellular region; basement membrane
Biological Process: epithelial cell proliferation; camera-type eye morphogenesis; angiogenesis; cell adhesion
Research Articles on COL8a1
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Product Notes
The COL8a1 col8a1 (Catalog #AAA2010877) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Collagen Type VIII Alpha 1 (COL8a1) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the COL8a1 col8a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type VIII Alpha 1 (COL8a1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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