Mouse anti-Human, Mouse DAB2IP Monoclonal Antibody | anti-DAB2IP antibody

DAB2IP Antibody

Cat. Num. AAA9216857

• Gene Names: DAB2IP; AIP1; AIP-1; AF9Q34; DIP1/2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: DAB2IP; Monoclonal Antibody; DAB2IP Antibody; Purified Mouse Monoclonal Antibody (Mab); Disabled homolog 2-interacting protein; DAB2 interaction protein; DAB2-interacting protein; ASK-interacting protein 1; AIP-1; DOC-2/DAB-2 interactive protein; AF9Q34; AIP1; KIAA1743; anti-DAB2IP antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Sequence Length: 1132

• Applicable Applications for anti-DAB2IP antibody: Western Blot (WB)
• Application Notes: WB~~1:1000
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-DAB2IP Antibody at 1:1000 dilutionLane 1: Du145 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: mouse brain lysateLane 4: Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 132 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-DAB2IP antibody

Functions as a scaffold protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Involved in several processes such as innate immune response, inflammation and cell growth inhibition, apoptosis, cell survival, angiogenesis, cell migration and maturation. Plays also a role in cell cycle checkpoint control; reduces G1 phase cyclin levels resulting in G0/G1 cell cycle arrest. Mediates signal transduction by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF), interferon (IFN) or lipopolysaccharide (LPS). Modulates the balance between phosphatidylinositol 3-kinase (PI3K)-AKT-mediated cell survival and apoptosis stimulated kinase (MAP3K5)-JNK signaling pathways; sequesters both AKT1 and MAP3K5 and counterbalances the activity of each kinase by modulating their phosphorylation status in response to proinflammatory stimuli. Acts as a regulator of the endoplasmic reticulum (ER) unfolded protein response (UPR) pathway; specifically involved in transduction of the ER stress-response to the JNK cascade through ERN1. Mediates TNF-alpha-induced apoptosis activation by facilitating dissociation of inhibitor 14-3-3 from MAP3K5; recruits the PP2A phosphatase complex which dephosphorylates MAP3K5 on 'Ser-966', leading to the dissociation of 13-3-3 proteins and activation of the MAP3K5-JNK signaling pathway in endothelial cells. Mediates also TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK-NF-kappa-B signaling. Acts a negative regulator in the IFN-gamma-mediated JAK-STAT signaling cascade by inhibiting smooth muscle cell (VSMCs) proliferation and intimal expansion, and thus, prevents graft arteriosclerosis (GA). Acts as a GTPase-activating protein (GAP) for the ADP ribosylation factor 6 (ARF6) and Ras. Promotes hydrolysis of the ARF6-bound GTP and thus, negatively regulates phosphatidylinositol 4,5- bisphosphate (PIP2)-dependent TLR4-TIRAP-MyD88 and NF-kappa-B signaling pathways in endothelial cells in response to lipopolysaccharides (LPS). Binds specifically to phosphatidylinositol 4-phosphate (PtdIns4P) and phosphatidylinositol 3-phosphate (PtdIns3P). In response to vascular endothelial growth factor (VEGFA), acts as a negative regulator of the VEGFR2-PI3K-mediated angiogenic signaling pathway by inhibiting endothelial cell migration and tube formation. In the developing brain, promotes both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex in a glial-dependent locomotion process. Probable downstream effector of the Reelin signaling pathway; promotes Purkinje cell (PC) dendrites development and formation of cerebellar synapses. Functions also as a tumor suppressor protein in prostate cancer progression; prevents cell proliferation and epithelial-to-mesenchymal transition (EMT) through activation of the glycogen synthase kinase-3 beta (GSK3B)-induced beta-catenin and inhibition of PI3K-AKT and Ras-MAPK survival downstream signaling cascades, respectively.

Product Categories/Family for anti-DAB2IP antibody

Crown Antibodies; Cancer

References

Chen H.,et al.Genomics 79:573-581(2002). von Bergh A.R.M.,et al.Genes Chromosomes Cancer 39:324-334(2004). Nagase T.,et al.DNA Res. 7:347-355(2000). Nakajima D.,et al.DNA Res. 9:99-106(2002). Humphray S.J.,et al.Nature 429:369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 94967023
• NCBI GeneID: 153090
• NCBI Accession #: NP_115941.2
• NCBI GenBank Nucleotide #: NM_032552.3
• UniProt Accession #: Q5VWQ8; Q8TDL2; Q96SE1; Q9C0C0; A6H8V2; A6NHI9; B0QZB1; G3XA90
• Molecular Weight: 125,041 Da
• NCBI Official Full Name: disabled homolog 2-interacting protein isoform 1
• NCBI Official Synonym Full Names: DAB2 interacting protein
• NCBI Official Symbol: DAB2IP
• NCBI Official Synonym Symbols: AIP1; AIP-1; AF9Q34; DIP1/2
• NCBI Protein Information: disabled homolog 2-interacting protein
• UniProt Protein Name: Disabled homolog 2-interacting protein
• Protein Family: Disabled homolog 2-interacting protein
• UniProt Gene Name: DAB2IP
• UniProt Synonym Gene Names: AF9Q34; AIP1; KIAA1743; DAB2 interaction protein; DAB2-interacting protein; AIP-1
• UniProt Entry Name: DAB2P_HUMAN

NCBI Description

DAB2IP is a Ras (MIM 190020) GTPase-activating protein (GAP) that acts as a tumor suppressor. The DAB2IP gene is inactivated by methylation in prostate and breast cancers (Yano et al., 2005 [PubMed 15386433]).[supplied by OMIM, May 2010]

Uniprot Description

DAB2IP: Functions as a Ras GTPase-activating protein. Through its interaction with MAP3K5 disrupts the association of MAP3K5 with the inhibitory 14-3-3 complex. Mediates TNF/TRAF2-induced MAP3K5-JNK activation, while it inhibits CHUK- NF-kappa-B signaling. May act as a tumor suppressor gene. A chromosomal aberration involving DAB2IP is found in a patient with acute myeloid leukemia (AML). Translocation t(9;11)(q34;q23) with MLL. May give rise to a MLL-DAB2IP fusion protein lacking the PH domain. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 9q33.1-q33.3

Cellular Component: axon; cell soma; cytoplasm; cytosol; dendrite; endocytic vesicle; plasma membrane

Molecular Function: death receptor binding; GTPase activator activity; identical protein binding; kinase binding; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; phosphatidylinositol 3-phosphate binding; phosphoinositide 3-kinase binding; protein binding; protein complex binding; protein homodimerization activity; protein kinase binding; protein phosphatase 2A binding; SH3 domain binding; vascular endothelial growth factor receptor 2 binding

Biological Process: activation of JNK activity; activation of MAPKK activity; activation of MAPKKK activity; angiogenesis; axon guidance; cell cycle; cell locomotion involved in cerebral cortex radial glia guided migration; cellular protein catabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; I-kappaB phosphorylation; induction of apoptosis via death domain receptors; inflammatory response; inhibition of NF-kappaB transcription factor; innate immune response; insulin receptor signaling pathway; layer formation in the cerebral cortex; lumen formation; MAPKKK cascade; negative regulation of angiogenesis; negative regulation of cell proliferation; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of fibroblast proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of MAP kinase activity; negative regulation of phosphoinositide 3-kinase activity; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of protein amino acid phosphorylation; negative regulation of Ras protein signal transduction; negative regulation of toll-like receptor 4 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of vascular endothelial growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; neurite morphogenesis; positive regulation of apoptosis; positive regulation of GTPase activity; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; Ras protein signal transduction; regulation of growth; regulation of GTPase activity; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of protein complex assembly; regulation of protein heterodimerization activity; small GTPase mediated signal transduction; transformed cell apoptosis; unfolded protein response, activation of signaling protein activity; vascular endothelial growth factor receptor signaling pathway