Rabbit anti-Human, Mouse SMURF1 Polyclonal Antibody | anti-SMURF1 antibody

SMURF1, NT (SMURF1, KIAA1625, E3 ubiquitin-protein ligase SMURF1, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1) (PE)

Cat. Num. AAA6349143

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SMURF1; Polyclonal Antibody; NT (SMURF1; KIAA1625; E3 ubiquitin-protein ligase SMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1) (PE); SMAD-specific E3 ubiquitin-protein ligase 1; anti-SMURF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SMURF1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74~104 amino acids from the N-terminal region of human SMURF1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SMURF1 Pab in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-SMURF1 antibody

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Product Categories/Family for anti-SMURF1 antibody

Antibodies; Ubiquitin Ligases

References

Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003). Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002). Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001). Zhu, H., et al., Nature 400(6745):687-693 (1999). Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).

NCBI and Uniprot Product Information

• NCBI GI #: 315434204
• NCBI GeneID: 57154
• NCBI Accession #: NP_001186776.1
• NCBI GenBank Nucleotide #: NM_001199847.1
• UniProt Accession #: Q9HCE7; O75853; Q547Q3; Q9UJT8; A4D279; B7ZMB6; B9EGV3
• Molecular Weight: 83,440 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase SMURF1 isoform 3
• NCBI Official Synonym Full Names: SMAD specific E3 ubiquitin protein ligase 1
• NCBI Official Symbol: SMURF1
• NCBI Protein Information: E3 ubiquitin-protein ligase SMURF1
• UniProt Protein Name: E3 ubiquitin-protein ligase SMURF1
• UniProt Gene Name: SMURF1
• UniProt Synonym Gene Names: KIAA1625; hSMURF1
• UniProt Entry Name: SMUF1_HUMAN

NCBI Description

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]

Uniprot Description

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: axon; cell soma; cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane

Molecular Function: activin binding; ligase activity; phospholipid binding; protein binding; ubiquitin-protein ligase activity

Biological Process: BMP signaling pathway; cell differentiation; ectoderm development; negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of defense response to virus by host; proteasomal ubiquitin-dependent protein catabolic process; protein export from nucleus; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway