GFRA3 sirna

GFRA3 siRNA (Mouse)

Cat. Num. AAA8227174

• Gene Names: Gfra3; Y15110; GFRalpha3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GFRA3; GFRA3 siRNA (Mouse); GDNF family receptor alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GFRA3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 397

• Applicable Applications for GFRA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GFRA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GFRA3 sirna

siRNA to inhibit GFRA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160298126
• NCBI GeneID: 14587
• NCBI Accession #: NP_034410.3
• NCBI GenBank Nucleotide #: NM_010280.4
• UniProt Accession #: O35118; O35325; O55243; Q6NZC2; Q8C8L9
• Molecular Weight: 44,307 Da
• NCBI Official Full Name: GDNF family receptor alpha-3 preproprotein
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 3
• NCBI Official Symbol: Gfra3
• NCBI Official Synonym Symbols: Y15110; GFRalpha3
• NCBI Protein Information: GDNF family receptor alpha-3
• UniProt Protein Name: GDNF family receptor alpha-3
• Protein Family: GDNF family receptor
• UniProt Gene Name: Gfra3
• UniProt Synonym Gene Names: GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
• UniProt Entry Name: GFRA3_MOUSE

NCBI Description

This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Cellular Component: intracellular membrane-bound organelle; membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; axon guidance receptor activity

Biological Process: axon guidance; sympathetic nervous system development; neuron migration; neuron development; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway