Typical Testing Data/Standard Curve (for reference only)
Mouse S Antigen ELISA Kit | SAG elisa kit
Mouse S Antigen ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SAG antibody and an SAG-HRP conjugate. The assay sample and buffer are incubated together with SAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAG concentration since SAG from samples and SAG-HRP conjugate compete for the anti-SAG antibody binding site. Since the number of sites is limited, as more sites are occupied by SAG from the sample, fewer sites are left to bind SAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAG concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]
Uniprot Description
SAG: Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Retina and pineal gland. Belongs to the arrestin family.
Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: photoreceptor outer segment; photoreceptor inner segment; cytosol
Molecular Function: opsin binding; phosphoprotein binding; protein phosphatase inhibitor activity
Biological Process: rhodopsin mediated signaling; phototransduction, visible light; cell surface receptor linked signal transduction; regulation of rhodopsin mediated signaling; visual perception; negative regulation of catalytic activity
Disease: Oguchi Disease 1; Retinitis Pigmentosa 47
Research Articles on SAG
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Product Notes
The Mouse SAG sag (Catalog #AAA731541) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA731541 ELISA Kit recognizes Mouse SAG. It is sometimes possible for the material contained within the vial of "S Antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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