Typical Testing Data/Standard Curve (for reference only)
Human Cytochrome P450 26B1 (CYP26B1) ELISA Kit | CYP26B1 elisa kit
Human Cytochrome P450 26B1 (CYP26B1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: CYP26B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CYP26B1 antibody and an CYP26B1-HRP conjugate. The assay sample and buffer are incubated together with CYP26B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP26B1 concentration since CYP26B1 from samples and CYP26B1-HRP conjugate compete for the anti-CYP26B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP26B1 from the sample, fewer sites are left to bind CYP26B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP26B1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein is localized to the endoplasmic reticulum, and functions as a critical regulator of all-trans retinoic acid levels by the specific inactivation of all-trans retinoic acid to hydroxylated forms. Mutations in this gene are associated with radiohumeral fusions and other skeletal and craniofacial anomalies, and increased levels of the encoded protein are associated with atherosclerotic lesions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]
Uniprot Description
CYP26B1: Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH- RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints. Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA). A disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects. Belongs to the cytochrome P450 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.-.-; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Cell development/differentiation
Chromosomal Location of Human Ortholog: 2p13.2
Cellular Component: endoplasmic reticulum membrane; cytoplasm
Molecular Function: retinoic acid binding; iron ion binding; retinoic acid 4-hydroxylase activity; heme binding
Biological Process: retinoic acid receptor signaling pathway; negative regulation of retinoic acid receptor signaling pathway; tongue morphogenesis; vitamin metabolic process; xenobiotic metabolic process; spermatogenesis; cell fate determination; male meiosis; proximal/distal pattern formation; embryonic limb morphogenesis
Disease: Radiohumeral Fusions With Other Skeletal And Craniofacial Anomalies
Research Articles on CYP26B1
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Product Notes
The Human CYP26B1 cyp26b1 (Catalog #AAA7210472) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7210472 ELISA Kit recognizes Human CYP26B1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 26B1 (CYP26B1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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