GFR Alpha-3/GFRA3 Recombinant Protein | GFRA3 recombinant protein

GFR Alpha-3/GFRA3 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123021

• Gene Names: Gfra3; Y15110; GFRalpha3
• Purity: >80% as determined by SDS-PAGE
• Synonyms: GFR Alpha-3/GFRA3; GFR Alpha-3/GFRA3 Protein; Mouse; Recombinant (hFc Tag); Mouse GFRA3/GFR-alpha-3 Protein (Fc Tag); GFRalpha3 Protein; Y15110 Protein; GDNF family receptor alpha 3; GFRA3 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg379

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 29
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse GFRA3 (O35118)(Met 1-Arg 379), was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GFRA3 recombinant protein

Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3/GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor alpha3 (GFRA3/GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.

References

Widenfalk J, et al. (2000) Neurturin, RET, GFRalpha-1 and GFRalpha-2, but not GFRalpha-3, mRNA are expressed in mice gonads. Cell Tissue Res. 299(3): 409-15.Li J, et al. (2009) Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice. FEBS Lett. 583(15): 2479-85.Yang C, et al. (2006) Distribution of GDNF family receptor alpha3 and RET in rat and human non-neural tissues. J Mol Histol. 37(1-2): 69-77.

NCBI and Uniprot Product Information

• NCBI GI #: 2627159
• NCBI GeneID: 14587
• UniProt Accession #: O35118; O35325; O55243; Q6NZC2; Q8C8L9
• Molecular Weight: 44,307 Da
• NCBI Official Full Name: Mus musculus mRNA for GFRalpha-3, complete cds
• NCBI Official Synonym Full Names: glial cell line derived neurotrophic factor family receptor alpha 3
• NCBI Official Symbol: Gfra3
• NCBI Official Synonym Symbols: Y15110; GFRalpha3
• NCBI Protein Information: GDNF family receptor alpha-3; GFR alpha-3; GFR-alpha-3; GDNFR-alpha-3; GDNF receptor alpha-3
• UniProt Protein Name: GDNF family receptor alpha-3
• Protein Family: GDNF family receptor
• UniProt Gene Name: Gfra3
• UniProt Synonym Gene Names: GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3
• UniProt Entry Name: GFRA3_MOUSE

NCBI Description

This gene encodes a cell surface glycoprotein and member of the glial cell line-derived neurotrophic receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein mediates binding of the ligand artemin to the ret receptor tyrosine kinase, and this interaction may regulate thermal pain and axon regeneration. Homozygous knockout mice for this gene exhibit impaired proliferation of cultured neuroblasts and impaired development of the superior cervical ganglion. [provided by RefSeq, Aug 2015]

Uniprot Description

GFRA3: Receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; receptor activity; axon guidance receptor activity

Biological Process: axon guidance; sympathetic nervous system development; neuron migration; neuron development; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway