SDS-Page
PRTFDC1 recombinant protein
PRTFDC1 Protein, Human, Recombinant (His Tag)
Gene Names
PRTFDC1; HHGP
Purity
>95% as determined by SDS-PAGE
Synonyms
PRTFDC1; PRTFDC1 Protein; Human; Recombinant (His Tag); Human PRTFDC1 Protein (His Tag); HHGP Protein; phosphoribosyl transferase domain containing 1; PRTFDC1 recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Val225
Species
Human
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the mature form of human PRTFDC1 (Q9NRG1-1)(Met1-Val225) was expressed with a polyhistide tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for PRTFDC1 recombinant protein
Background: PRTFDC1 is a member of the purine/pyrimidine phosphoribosyltransferase family. It can bind GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). The epigenetic silencing of PRTFDC1 by hypermethylation of the CpG island leads to a loss of PRTFDC1 function, which might be involved in squamous cell oral carcinogenesis. PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse and has important implications for unraveling the molecular etiology of lesch-Nyhan disease(LND). LND is a severe X-linked neurological disorder caused by a deficiency of hypoxanthine phosphoribosyltransferase. PRTFDC1 has a low, barely measurable phosphoribosyltransferase activity (in vitro).
References
Welin M. et al., 2010, FEBS J. 277 (23): 4920-30. Keebaugh AC. et al., 2011, PLoS One. 6 (7): e22381. Suzuki E. et al., 2007, Oncogene. 26 (57): 7921-32.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,983 Da
NCBI Official Full Name
phosphoribosyltransferase domain-containing protein 1 isoform 2
NCBI Official Synonym Full Names
phosphoribosyl transferase domain containing 1
NCBI Official Symbol
PRTFDC1
NCBI Official Synonym Symbols
HHGP
NCBI Protein Information
phosphoribosyltransferase domain-containing protein 1
UniProt Protein Name
Phosphoribosyltransferase domain-containing protein 1
Protein Family
UniProt Gene Name
PRTFDC1
UniProt Synonym Gene Names
HHGP
UniProt Entry Name
PRDC1_HUMAN
Uniprot Description
PRTFDC1: Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage. Belongs to the purine/pyrimidine phosphoribosyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: cytosol
Molecular Function: protein homodimerization activity; nucleotide binding; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding
Biological Process: GMP catabolic process; hypoxanthine metabolic process; IMP salvage; GMP salvage; adenine salvage; purine ribonucleoside salvage; guanine salvage
Research Articles on PRTFDC1
Similar Products
Product Notes
The PRTFDC1 prtfdc1 (Catalog #AAA8121613) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Val22 5. It is sometimes possible for the material contained within the vial of "PRTFDC1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
