Mouse anti-Human PRTFDC1 Monoclonal Antibody | anti-PRTFDC1 antibody

Anti-PRTFDC1 Antibody, Mouse Monoclonal

Cat. Num. AAA8107218

• Gene Names: PRTFDC1; HHGP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: PRTFDC1; Monoclonal Antibody; Anti-PRTFDC1 Antibody; Mouse Monoclonal; PRTFDC1 Antibody; Mouse MAb; phosphoribosyl transferase domain containing 1; Anti-HHGP Antibody; anti-PRTFDC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human PRTFDC1.; No cross-reactivity in ELISA with E Coli cell lysate
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRTFDC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Human PRTFDC1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PRTFDC1 (rh PRTFDC1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PRTFDC1 mouse monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:28 kDaObserved band size:28 kDa)

Related Product Information for anti-PRTFDC1 antibody

PRTFDC1 is a member of the purine/pyrimidine phosphoribosyltransferase family. It can bind GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). The epigenetic silencing of PRTFDC1 by hypermethylation of the CpG island leads to a loss of PRTFDC1 function, which might be involved in squamous cell oral carcinogenesis. PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse and has important implications for unraveling the molecular etiology of lesch-Nyhan disease(LND). LND is a severe X-linked neurological disorder caused by a deficiency of hypoxanthine phosphoribosyltransferase. PRTFDC1 has a low, barely measurable phosphoribosyltransferase activity (in vitro).

References

Welin M. et al., 2010, FEBS J. 277 (23): 4920-30. Keebaugh AC. et al., 2011, PLoS One. 6 (7): e22381. Suzuki E. et al., 2007, Oncogene. 26 (57): 7921-32.

NCBI and Uniprot Product Information

• NCBI GI #: 544346261
• NCBI GeneID: 56952
• NCBI Accession #: NP_001269715.1
• NCBI GenBank Nucleotide #: NM_001282786.1
• UniProt Accession #: Q9NRG1; Q53HA7; Q59EL9; Q5VV18; Q5VV20; B7Z1Z3
• Molecular Weight: 19,983 Da
• NCBI Official Full Name: phosphoribosyltransferase domain-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: phosphoribosyl transferase domain containing 1
• NCBI Official Symbol: PRTFDC1
• NCBI Official Synonym Symbols: HHGP
• NCBI Protein Information: phosphoribosyltransferase domain-containing protein 1
• UniProt Protein Name: Phosphoribosyltransferase domain-containing protein 1
• Protein Family: Phosphoribosyltransferase domain-containing protein
• UniProt Gene Name: PRTFDC1
• UniProt Synonym Gene Names: HHGP
• UniProt Entry Name: PRDC1_HUMAN

Uniprot Description

PRTFDC1: Has low, barely detectable phosphoribosyltransferase activity (in vitro). Binds GMP, IMP and alpha-D-5-phosphoribosyl 1-pyrophosphate (PRPP). Is not expected to contribute to purine metabolism or GMP salvage. Belongs to the purine/pyrimidine phosphoribosyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; nucleotide binding; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding

Biological Process: GMP catabolic process; hypoxanthine metabolic process; IMP salvage; GMP salvage; adenine salvage; purine ribonucleoside salvage; guanine salvage