Western Blot (WB)
(Anti-PELO rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PELO/pelota homolog Polyclonal Antibody | anti-PELO antibody
Anti-PELO/pelota homolog Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PELO/pelota homolog; Polyclonal Antibody; Anti-PELO/pelota homolog Antibody; Rabbit Polyclonal; PELO/pelota homolog Antibody; Rabbit PAb; Antigen Affinity Purified; pelota homolog (Drosophila); Anti-CGI-17 Antibody; Anti-PRO1770 Antibody; anti-PELO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PELO/pelota homolog
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PELO antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human PELO/pelota homolog fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PELO/pelota homolog fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PELO rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PELO rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-PELO antibody
PELO (Pelota MRNA Surveillance And Ribosome Rescue Factor) is a Protein Coding gene. The PELO gene, located on 5q11.2, is conserved in dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, and S.pombe. This gene encodes a protein that contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and meiotic cell division. The pelota gene of Drosophila melanogaster encodes a protein that was found to be included in cell cycle regulation. Mutations were found to result in spermatogenic arrest, female sterility, and disturbances in the patterning of the eye. Diseases associated with PELO include Angiokeratoma Of Fordyce. Among its related pathways are Focal Adhesion and mRNA surveillance pathway.
References
Shamsadin R, et al. (2002) Mouse pelota gene (pelo): Cdna cloning, genomic structure, and chromosomal localization. Cytogenet Genome Res 97 (1-2): 95-99.Shamsadin R, et al. (2000) Molecular cloning, expression and chromosome location of the human pelota gene pelo. Cytogenet Cell Genet 90 (1-2): 75-78.Raju P, et al. (2015) Pelota mediates gonocyte maturation and maintenance of spermatogonial stem cells in mouse testes. Reproduction 149 (3): 213-221.Burnicka-Turek O, et al. (2010) Pelota interacts with hax1, eif3g and srpx and the resulting protein complexes are associated with the actin cytoskeleton. BMC Cell Biol 11 28.
Similar Products
Product Notes
The PELO (Catalog #AAA8108605) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PELO/pelota homolog Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PELO/pelota homolog can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PELO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PELO/pelota homolog, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
