Rabbit anti-Human ACOT13/THEM2 Polyclonal Antibody | anti-ACOT13 antibody

Anti-ACOT13/THEM2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107585

• Gene Names: ACOT13; HT012; THEM2; PNAS-27
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ACOT13/THEM2; Polyclonal Antibody; Anti-ACOT13/THEM2 Antibody; Rabbit Polyclonal; THEM2/ACOT13 Antibody; Rabbit PAb; Antigen Affinity Purified; acyl-CoA thioesterase 13; Anti-ACOT13 Antibody; Anti-HT012 Antibody; Anti-PNAS-27 Antibody; Anti-THEM2 Antibody; anti-ACOT13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human THEM2/ACOT13
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ACOT13 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human THEM2/ACOT13 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human THEM2/ACOT13 (rh THEM2/ACOT13). THEM2/ACOT13 specific IgG was purified by Human THEM2/ACOT13 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-THEM2 rabbit polyclonal antibody at 1:500 dilution Lane A: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:15 kDa Observed band size:15 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human THEM2 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human THEM2 in human hepatoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(THEM2 was immunoprecipitated using: Lane A:0.5 mg Mouse liver tissue lysate4 uL anti-THEM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody: Anti-THEM2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.Predicted band size: 15 kDa Observed band size :15 kDa)

Related Product Information for anti-ACOT13 antibody

The metabolites of fatty acyl-Coenzyme A (CoA) and metabolic enzymes contribute to lipid biosynthesis, signal transduction, and gene transcription. Members of the acyl-CoA thioesterase (Acot) gene family hydrolyze fatty acyl-CoAs, but their biological functions remain incompletely understood. Thioesterase superfamily member 2 (Them2; synonym Acot13) is enriched in oxidative tissues, associated with mitochondria, and relatively specific for long chain fatty acyl-CoA substrates. Using Them2(-/-) mice, we have demonstrated key roles for Them2 in regulating hepatic glucose and lipid metabolism. Members of the acyl-CoA thioesterase (Acot) gene family catalyze the hydrolysis of fatty acyl-CoAs, but their biological functions remain unknown. Thioesterase superfamily member 2 (Them2; synonym Acot13) is a broadly expressed mitochondria-associated Acot. Them2 was previously identified as an interacting protein of phosphatidylcholine transfer protein (PC-TP).

NCBI and Uniprot Product Information

• NCBI GI #: 8923812
• NCBI GeneID: 55856
• NCBI Accession #: NP_060943.1
• NCBI GenBank Nucleotide #: NM_018473.3
• UniProt Accession #: Q9NPJ3
• NCBI Official Full Name: acyl-coenzyme A thioesterase 13 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 13
• NCBI Official Symbol: ACOT13
• NCBI Official Synonym Symbols: HT012; THEM2; PNAS-27
• NCBI Protein Information: acyl-coenzyme A thioesterase 13
• UniProt Protein Name: Acyl-coenzyme A thioesterase 13
• Protein Family: Acyl-coenzyme A thioesterase
• UniProt Gene Name: ACOT13
• UniProt Synonym Gene Names: THEM2; Acyl-CoA thioesterase 13
• UniProt Entry Name: ACO13_HUMAN

NCBI Description

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Uniprot Description

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family.

Protein type: EC 3.1.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrion; spindle; nucleus; cytosol

Molecular Function: acyl-CoA hydrolase activity

Biological Process: metabolic process; protein homotetramerization